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A member asked:
What does a high positive elisa but negative ana mean for a lupus screening? how are they different and how can 1 be positive and one negative?
A doctor has provided 1 answer
ANA: 1. ELISA is an immunoassay used to screen for ANA (high sensitivity, relatively low specificity). Can have false positive even with high titer. 2.If ELISA positive, usually a confirmation test will follow. Indirect Fluorescent Antibody Assay (IFA) is more specific test used as a golden standard to detect ANA. Hope that helps.
Answered 8/31/2014
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