Can bilateral l5 nerve root compression from grade 2 spondylolisthesis cause consistent muscle twitches/fasciculations in calves, top of foot, arch of foot, and the tibialis anterior muscle?

The simple answer to your question is yes. The L5 nerve root is part of the sciatic nerve innervating the lower extremities. I would want to ask you why such a young person has such pathology to the lower back? Nerve conduction studies can diagnose the problem. A neurologist can help diagnose the problem. A neurosurgeon or orthopedic surgeon would have to be considered for any type of procedures

Yes, if the nerve roots are substantially compressed, the muscles innervated would demonstrate secondary issues. But you do mention involvement in some muscles in the S-1 distribution, so, an EMG would be instructive. Perhaps a question of surgery would be salient if significant denervation were noted on electrodiagnostic testing.