What does a variation in results for ana blood test in the past all were negative what does it mean if the titer is 1:80 and a speckled pattern ?

Not much: You need tests for specific antibodies in order to diagnose an auto-immune inflammatory condition such as lupus. See your doctor for complete evaluation. ANA has a high incidence of false positives.

Autoimmune disease: ANA is a marker for autoimmune diseases like lupus and rheumatoid arthritis. A <1:80 titer is low, meaning there is a lower chance of disease. A "speckled pattern" refers to the staining pattern of the markers, which is seen under the microscope. Certain autoimmune diseases produce specific staining patterns. A negative ANA means you do not have that marker of disease.