A member asked:

I have to take a elisa test today for lyme disease but i was just wondering how does elisa test work?

A doctor has provided 1 answer
Dr. Kathleen Mullane answered

Specializes in Internal Medicine

Sandwich assay...yum: Elisa = enzyme-linked immuno sorbent assay. Done by adding serum from a subject to a plate w/antibody against the infection you're testing for on its surface. The antigen (or protein) from the infection binds to the antibody on the plate. Then another antibody to the infection w/color signal chemical attached is added on top and binds to the antigen. If infection is there color can be measured.

Answered 4/20/2013

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