Sir my husband has a doubt on me he is asking a medical certificate hat i have not committed mistake related to sex, is any any test regarding this.

No: There is no test to prove that you have had sex in the past. Even a physical exam cannot be certain. Tell your husband that this physician recommends he start trusting you if he wants to have a good marriage. A GOOD man will honor and respect a woman. A man who cannot, does not deserve to have a wife.

No: Unfortunately there is no such certificate to prove you haven't had sex outside marriage. Your husband seems to have trust issues with you and what you both need is marital counselling