Is possible to have both vitamin d tests drawn in one blood lab?

Yes, 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D levels can be assessed by laboratories. Although there are differences among Vitamin D methods, most laboratories utilize similar reference intervals. Because toxicity is rare, the focus has been on the lower limit and what cut-off for total 25-hydroxyvitamin D (D2 + D3) indicates deficiency [usually below 20 ng/mL (normal 30-50 nmol/liter)].