This is a ridiculous question, but if a shirt as been washed and dried more than twice, is it possible for the brain eating amoeba to survive on the shirt? i have ocd so i know its an irrational question?

Naegleria fowleri infects people when water containing the ameba enters the body through the nose. This typically happens when people go swimming, diving, or when they put their heads under fresh water, like in lakes and rivers. Your shirt poses zero risk. ref: https://www.cdc.gov/parasites/naegleria/general.html#

Hopefully, your clean shirt will not be a source of any infectious organism, and amoeba typically don't survive washing machines. However, your emotional distress would stabilize if you were to receive cognitive-behavioral therapy and biofeedback therapy.