A member asked:

What is the muscle staining process to diagnose muscular dystrophy?

A doctor has provided 1 answer
Dr. Saptarshi Bandyopadhyay answered

Specializes in Hospital-based practice

Biopsy; NADH: The 1st step is to obtain a muscle biopsy from (usually) a large muscle fiber (quads, thigh, foreleg) but it can vary depending on symptoms. Then the biopsy is stained with contrast for diagnosis (to differentiate the muscle from fat & other tissue). NADH & acid phosphatase stains are usually used but others are possible (Sudan, etc). Diagnosis depends on the pattern of what the microscope shows.

Answered 4/7/2016

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