What is the muscle staining process to diagnose muscular dystrophy?

Biopsy; NADH: The 1st step is to obtain a muscle biopsy from (usually) a large muscle fiber (quads, thigh, foreleg) but it can vary depending on symptoms. Then the biopsy is stained with contrast for diagnosis (to differentiate the muscle from fat & other tissue). NADH & acid phosphatase stains are usually used but others are possible (Sudan, etc). Diagnosis depends on the pattern of what the microscope shows.