Vaginal itching, been tested 4 all stds more than 1x,had multiple cultures done, all neg., tried antibiotics/yeast treatments,they didn't help?

More info: More info, such as presence of discharge or where the symptoms are, could help me understand what's going on. Also, your age would be very useful.

Need thorough evaluation: You need a thorough evaluation. If you are perimenopausal, decreased estrogen levels can make the vaginal and vulvar tissue thinner, so more prone to irritation. It could be a reaction to a soap or other chemical you are exposed to. If it's always one spot, it may need to be examined for a skin disorder, such as lichen sclerosis or vulvar or vaginal neoplasia.