From one time of doing chewing tobacco, can i get mouth cancer?

Technically yes: The more you dip the greater are your chances.

No: You should be fine. Smoking and chewing are both unhealthy habits that can have horrible consequences so do yourself a favor and don't ever get into the habit. Hope this helps.

No, but: No, but why start? See oral surgeon or ENT doc if you have any of these symptoms: Persistent mouth sore, persistent mouth pain, a lump or thickening in the cheek, a white/red patch on the gums, tongue, tonsil, or lining of the mouth, a sore throat, hoarseness or feeling that something is caught in the throat that does not go away, difficulty swallowing/chewing.