Hi doctor, can masturbation twice of thrice a weak can cause hand shake . i am very worried and i am just 24. please help. ?

No. See a doctor: Masturbation does not have significant adverse health effects and certainly does not cause hand shaking (though some will shake their hands as part of the act of masturbating, depending on technique). The most common cause of hands shaking in a 24 yo is Benign Essential Tremor, but could also be related to heavy metal toxicity, Lyme disease etc. See a good Dr. Consider also consulting a homeopath.