Do you have to have a prescription for polysporin (bacitracin and polymyxin) eyedrops?

Yes: See a physician before apllying any antibiotics into the eye.

In the U.S., yes: Might be different in other countries, but in the U.S., the answer is yes, you need a prescription. There are many causes of eye irritation and you really don't want to be putting antibiotic drops in your eyes unless infection is truly the cause of your problem. Don't take a chance with your vision!