A 45-year-old member asked:
Could you please describe the process of making monoclonal antibodies and what is their purpose?
1 doctor answer
Dr. Myron Arlen answered
63 years experience in Surgical Oncology
Hibidoma formation: Technique of Milstein where protein injected in a mouse. Spleen cells removed and B cells separated. The B cell producing antibody A is fused with a malignant antibody, the new cell type having a fused nucleus and producing one antibody. This new cell is immortalized due to the malignant component and is a hybrid cell called a hybridoma producing an antibody from one clone known as a monoclonal
Answered on Jun 1, 2014
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