OPK: If the 1st 3 tests were negative and the last was positive, the last confirms that you are ovulating. Ovulation should occur within 24 - 36 hours. I would continue to monitor your next cycle to see if the kit will confirm your ovulation with each cycle so that you can get a better sense of when this occurs within your cycle. If you are trying to get pregnant, you should have sex around this time.
Answered 1/11/2014
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