Can one shot of whiskey show on a hair follicle drug screen?

Unlikely, but: it depends on several factors. Hair follicle drug screens are affected by: 1. time between consumption and hair collection, 2. type of hair collected (from scalp or body, as growth rates differ), 3. type of test -- the "standard or 5-panel" test generally does not include a test for alcohol. Consuming only "one shot" or 1-2 ounces of hard alcohol probably is not enough to cause a positive result