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A member asked:
What is the definition or description of: hiv 1/hiv 2 western blot?
A doctor has provided 1 answer
Immunoblot: Western blot is an antibody detection test. Viral antigens (the protein coat of hiv) are immobilized in a matrix (gel). These are then transferred to Cellulose membrane and a persons serum is exposed to it. Antibodies in the serum then bind to the proteins (ie gp120). Antibodies that do not attach are washed away. Chemicals are then applied to allow visualization of the bands.
Answered 6/24/2014
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