Is it safe to take .25mg of alprazolam 3 hours after a glass of wine?

No no! : alcohol and benzodiazepines can be a lethal combination. You will not get a physician on here to give you the OK to take the pill any periodof time after drinking alcohol. That discussion should be had between you and your primary care physician that prescribed you that medication. Keep in the back of your mind by mixing those two it can be lethal and I have seen it myself multiple times.