Does it matter whether practitioner is male or female for rolfing?

Depends: Most males have larger muscle structure so may be more effective. Beyond that is personal prefence - women often prefer women and men often prefer men. Look at training and experience as that is what counts beyond your personal preference.

Ida P. Rolf: The creator of rolfing (structural integration) was a woman named dr. Ida p. Rolf. Rolfing practioners can be men or women.