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What is the principle behind the occult blood test?

3 doctors weighed in across 2 answers

More than one: Guaiac test measures peroxidase activity which is associated with blood from any site in the GI tract, including blood in meats, and peroxidase in horse raddish. A different test measure human globin part of hemoglobin and detects only blood originating in the large intestine.

Answered 6/10/2014

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FIT or hemoccult?: If you're using the old hemoccult slide, you'll notice its two-sided. Place your specimen on one side, close the lid. Doctor or lab, when developing your specimen, turns the card over, opens the back & applies the developer solution which soaks thru to your specimen. A color change indicates your specimen contains invisible blood. Principle & mechanics differ with FIT (fecal immunochemical test).

Answered 10/31/2014

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