When is the best time to take a pregnancy test? i was told when you first wake up.?

Doesn't matter...: It doesn't matter what time of day one takes a pregnancy test, unless one is testing too soon. If one is patient and waits until a few days after missing a period, the HCG level should be high enough to cause a positive test at any hour. If testing too soon, a more concentrated urine sample (such as upon waking up) might be positive, while a more dilute sample later in the day might be negative.