3 main lab tests. Virus isolation (the most definitive, but results may take 1-2 wks), recently reverse transcriptase polymerase chain reaction has been developed (results within 1-2 days), & elisa serological tests requiring both an acute phase serum & convalescent phase serum 10-14 d after ds onset. Instructions for sending diagnostic specimens to cdc’s arbovirus diagnostic lab: —>see comment.
Yes. Igm antibody levels highest 3- 5 weeks after onset of illness & persist for 2 months. False positives occur with infection via related viruses, like o'nyong'nyong virus or semliki forest virus. Rt-pcr using nested primer pairs can amplify several chikungunya-specific genes from whole blood w/ results in 1-2 days but joint pain, conjunctivitis, insomnia, loss of taste often captures diagnosis too.