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I have to take a ELISA test today for Lyme disease but i was just wondering how does ELISA test work?

2 doctors weighed in
Dr. Kathleen Mullane
Internal Medicine
1 doctor agrees

In brief: Sandwich assay...yum

Elisa = enzyme-linked immuno sorbent assay.
Done by adding serum from a subject to a plate w/antibody against the infection you're testing for on its surface. The antigen (or protein) from the infection binds to the antibody on the plate. Then another antibody to the infection w/color signal chemical attached is added on top and binds to the antigen. If infection is there color can be measured.

In brief: Sandwich assay...yum

Elisa = enzyme-linked immuno sorbent assay.
Done by adding serum from a subject to a plate w/antibody against the infection you're testing for on its surface. The antigen (or protein) from the infection binds to the antibody on the plate. Then another antibody to the infection w/color signal chemical attached is added on top and binds to the antigen. If infection is there color can be measured.
Dr. Kathleen Mullane
Dr. Kathleen Mullane
Thank
1 comment
Dr. Arthur Mandelin
http://en.wikipedia.org/wiki/ELISA
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