What is the relation between masturbating and testosterone?

Probable Relation. Testosterone is a known libido {desire} enhancer. What you do with your desire is a personal choice. Testosterone has other non-libido bodily functions as well.
See below. Masturbation may be associated with a decrease in testosterone levels. The clinical significance of this is unclear. Endocrine response to masturbation-induced orgasm in healthy men following a 3-week sexual abstinence. http://www.ncbi.nlm.nih.gov/pubmed/11760788?ordinalpos=16&itool=entrezsystem2.pentrez.pubmed.pubmed_resultspanel.pubmed_rvdocsum.