Does having intercourse prior to taking a pregnancy test affect the results?

No. A pregnancy test (on urine) measures the presence of HCG (human choriionic gonadotropin) in the urine that is present because of pregnancy. It is not a measure of semen or of anything in the vagina of anything to do with the immediate act of intercourse.
No. No, urine pregnancy tests rely on a chemical - HCG - which is present only with a developing baby. Nothing in semen or sperm should affect the test.

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