My daughter has ear tubes. Her ear canal is red and she is playing with it. Is it an ear infection.?

Maybe,maybe not. The canal is normally pinkish. The only way to really know is to have someone look at it. If tubes are in & an ear is infected, there is commonly pus draining into the canal.If the tube has a mucous plug in its window, the pus would not drain.
Possibly. Tubes do not prevent ear infections, despite the common misconception to that effect. That said, if the canal is inflamed, it can sometimes indicate an infection, so have her seen. You do not mention her age, but some children will have a tendency to play with their ears, just because they find them. Treat her for fever if she develops one, and have her seen.