Does driving at night affect my eye sight?

Driving. Not really. Some people will notice that their eyes will dry during prolonged driving. If so, use some artificial tears such as theratears for temporary relief. Take a break every hour or two and walk around at a rest stop if your eyes feel tired. Do not point the air vents toward your eyes, since they will dry out.
No. Driving at night, which is the equivalent of doing work in darker situations, will have no effect on the power or health of the eyes. If you are struggling while driving at night, see your ophthalmologist to see if there is some cause for this.
No. Low light does not have a negative effect on eyesight, but as the lens ages and become cloudy (cataract), glare from oncoming headlights can make driving difficult or even dangerous.