Used a whole bottle with tears naturale, (artificial tears) should this be refilled?

How to refill? Do you mean that you should get more ? If it worked, and your eyes are dry and need the extra lubrication, then by all means get more. If you mean can the bottle be refilled, i don't know how you would do this. Larger reservoir bottles of tears naturale (artificial tears) are not for sale. The bottle you emptied in use is disposable. Tears naturale (artificial tears) is a fine product and very useful.