Biopsy or blood test. Leishmania (kala-azar) is best diagnosed by taking a careful history, including travel to areas where leishmania is found. Many tests are avaialble, choice will depend upon specific symptoms. Consult an infectious disease specialist to help. Tests include: biopsy of spleen, bone marrow, lymph node or skin. Blood test include immnunofloursent assays, PCR www. Ncbi. Nlm. Nih. Gov/pubmedhealth/pmh0002362/.