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What does a high positive ELISA but negative ANA mean for a Lupus Screening? How are they different and how can 1 be positive and one negative?

1 doctor weighed in
Dr. Le Wang
Internal Medicine

In brief: ANA

1. ELISA is an immunoassay used to screen for ANA (high sensitivity, relatively low specificity).
Can have false positive even with high titer. 2.If ELISA positive, usually a confirmation test will follow. Indirect Fluorescent Antibody Assay (IFA) is more specific test used as a golden standard to detect ANA. Hope that helps.

In brief: ANA

1. ELISA is an immunoassay used to screen for ANA (high sensitivity, relatively low specificity).
Can have false positive even with high titer. 2.If ELISA positive, usually a confirmation test will follow. Indirect Fluorescent Antibody Assay (IFA) is more specific test used as a golden standard to detect ANA. Hope that helps.
Dr. Le Wang
Dr. Le Wang
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