I have needle like pain in vagina. I am not pregnant. I don't have any vaginal infection, urinary infection. I am not able to keep relations wid my husbnd?

Pelvic exam needed. It is virtually impossible to appropriately examine the vaginal vault and surrounding structures on your own due to basic anatomy. It's best if your are seen by a physician who will be able to exam you and perform a pelvic exam.
Be seen. There are many different possible causes, and your doctor can determine what is happening.