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Could you please describe the process of making monoclonal antibodies and what is their purpose?

1 doctor weighed in
Dr. Myron Arlen
Surgery - Oncology

In brief: Hibidoma formation

Technique of Milstein where protein injected in a mouse.
Spleen cells removed and B cells separated. The B cell producing antibody A is fused with a malignant antibody, the new cell type having a fused nucleus and producing one antibody. This new cell is immortalized due to the malignant component and is a hybrid cell called a hybridoma producing an antibody from one clone known as a monoclonal

In brief: Hibidoma formation

Technique of Milstein where protein injected in a mouse.
Spleen cells removed and B cells separated. The B cell producing antibody A is fused with a malignant antibody, the new cell type having a fused nucleus and producing one antibody. This new cell is immortalized due to the malignant component and is a hybrid cell called a hybridoma producing an antibody from one clone known as a monoclonal
Dr. Myron Arlen
Dr. Myron Arlen
Thank
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