For how long does it take tinactin (tolnaftate) athlete's foot spray to start working?

1-2 weeks . If you are not seeing any improvement with tinactin (tolnaftate) in 1-2 weeks, you should consider another antifungal medication such as Lamisil at, lotrimin (or generic equivalent). You should use the topical medication for approx. 4 weeks to be most effective. If severe, use of an oral antifungal is very effective. You would need to see your physician for this.