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Doctor insights on: Recombinant Dna Technology

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Dna (Definition)

Dna are the coding molecules found in all living things on earth which contain the instructions on how to build living things. Its is comprised the nucleotide molecules which are ...Read more


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How is DNA mutated by carcinogens ?

How is DNA mutated by carcinogens ?

DNA breakdown: Carcinogens can cause cells to rapidly divide in response to the continual attack on their survival. With this high cell turnover coves oxidative/free radical damage (stealing of electrons) and in many case errors in dna sequencing resulted in a mutated dna stand. The body sees this imperfect dna strand as a foreign invader and eliminates it through the immune system. ...Read more

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Which method of prenatal testing analyzes chromosomes?

Which method of prenatal testing analyzes chromosomes?

Invasive: The two methods for testing fetal chromosomes are chorionic villus sampling and amniocentesis. There is research on obtaining fetal dna from maternal blood, but that is not yet in widespread use. ...Read more

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Please interpret- C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

Please interpret-
C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

You have chlamydia: "Detected" means the test found chlamydia -- i.e. a positive result. Return to the doctor or clinic where the test was done for treatment. (If you tested yourself at a lab, see a doctor or clinic ASAP.) Your current or recent sex partner(s) also need treatment. Good luck! ...Read more

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Etiology of DNA mutations?

Etiology of DNA mutations?

Replication error: Most changes in dna come from errors in the duplication of the dna. But changes can occur in the structure from radiation and some chemicals especially those used in chemotherapy. Most mutations are harmless, some are tracked down by your normal reparative mechanism but a few can cause trouble including cancer. ...Read more

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How do dna, mutation, genotype, phenotype, and natural selection interconnect to cause evolution?

How do dna, mutation, genotype, phenotype, and natural selection interconnect to cause evolution?

Modern synthesis: It's as well-established as anything in science, but would take more than 400 characters to explain. You owe it to yourself, as an educated person who must participate in community decision making, to understand it. There are a huge number of good books on the subject. Best wishes. ...Read more

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How much DNA is needed for nanopore sequencing?

How much DNA is needed for nanopore sequencing?

A future scientist: How much do you think is required? a single strand? A single molecule? I encourage you to find the answer. Best of wishes to your future endeavors. ...Read more

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Qiagen hybrid capture ii, HPV .Are these tests for cervical cancer available in usa?

Qiagen hybrid capture ii, HPV .Are these tests for cervical cancer available in usa?

Yes!: HPV testing is commonly done as part of the pap smear screening for Cervical cancer. So most gynecologists get this done easily though a lab...the test was developed by Qiagen but now available all over the world. ...Read more

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What amount time/days are required for paternal genome and blastocyst development after embryo transfer?

What amount time/days are required for paternal genome and blastocyst development after embryo transfer?

What?: I'm not sure what your question is. 1. If you had a day 3 embryo transfer it will be a blastocyst in 2 days. If you had a day 5 transfer it is already a blastocyst. 2. Paternal genome is working from day one of combination of the two genomes. The dominant genes regardless of maternal or paternal origin take the lead. Sex development is initiated near the 9th week. ...Read more

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Apart from elisa, which other immunoassay(s) can quantitative antigen analysis use?

Apart from elisa, which other immunoassay(s) can quantitative antigen analysis use?

See below: Rocket electrophoresis, radial immunodiffusion, conventional radio-immunoassy, densitometric scanning. There are many variations of ELISA itself. ...Read more

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How much time/days are required for paternal genome and blastocyst development after embryo transfer?

How much time/days are required for paternal genome and blastocyst development after embryo transfer?

What?: I'm not sure what your question is. 1. If you had a day 3 embryo transfer it will be a blastocyst in 2 days. If you had a day 5 transfer it is already a blastocyst. 2. Paternal genome is working from day one of combination of the two genomes. The dominant genes regardless of maternal or paternal origin take the lead. Sex development is initiated near the 9th week. ...Read more

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Which of these 3 hep c antibody screening tests(methodology)is the most accurate: Architect Anti-HCV, Advia Centauer HCV or Roche Eclia?

Which of these 3 hep c antibody screening  tests(methodology)is the most accurate: Architect Anti-HCV,  Advia Centauer HCV or Roche Eclia?

About equal: All three methods are FDA approved and about equally accurate. If you have concern about active Hep C infection, get a Hep C nucleic acid test. For good health - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form. Practice safe sex. ...Read more

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Chemicals, Toxins, illegal/legal drugs. Do they teach that all of these have a DNA sequence? ...learning about DNA, Protein, Antibodies/Antigens,ect

Chemicals, Toxins, illegal/legal drugs. Do they teach that all of these have a DNA sequence? ...learning about DNA, Protein, Antibodies/Antigens,ect

No: The chemicals you mentioned are not DNA. Some toxins are proteins, others are different chemicals. The gene controlling the proteins have a DNA sequence but the proteins and chemicals do not. ...Read more

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Gene expression profiling predicts clinical outcome of breast cancer?

Gene expression profiling predicts clinical outcome of breast cancer?

We're Getting There: In the past, we used anatomic staging alone (tumor size, lymph node involvement) to guide rx. Gene profiling allows us a completely separate measure of prognosis, and, more importantly, can predict who may or may not benefit from different chemotherapy agents. In the future, we will hopefully be able to tailor our rx to the specific cancer being treated: the elusive personalized medicine. ...Read more

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Is hiv-1-pcr quantitative and HIV RNA PCR quantitative same tests?can DNA PCR test be quantitative?

Is hiv-1-pcr quantitative and HIV RNA PCR quantitative same tests?can DNA PCR test be quantitative?

HIV testing: Yes. Viral testing can be qualitative or quantitative. Most HIV RNA testing is quantitative. ...Read more

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Describe microrna gene expression deregulation in human breast cancer?

Describe microrna gene expression deregulation in human breast cancer?

Can't do in 400: The study of micro-rnas is cutting-edge and extremely arcane and you need to go directly to pubmed or one of the other big ways to access the scientific data. If someone is offering a breast cancer treatment to re-regulate your micro-rna's, call your district attorney. Glad you have an inquiring mind and i wish you luck with your project. ...Read more

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Explain why the accuracy of RNA transcription is not critical as the accuracy of DNA replication?

DNA is THE proof: In dna & RNA hierarchy , the dna the master replicates, basis of inheritance ( and propagation) RNA replicates particular segment, as gene expression & proof reading mechanism, copied so the replication of dna is important than RNA transcription. ...Read more