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Doctor insights on: Polymérase Chain Reaction

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How many types of pcr(polymerase chain reaction) available?

How many types of pcr(polymerase chain reaction) available?

Probably one.: In the rt-pcr test, viral RNA is extracted from the patient's plasma and treated with reverse transcriptase (rt) to convert the viral RNA into cdna. The polymerase chain reaction (pcr) process is then applied. This is then quantified. There is a quantiplex bdna or branched dna test, but it is done by centrifuge to concentrate and detect by a color reaction that can be quantified, not pcr. ...Read more

Reaction To Hib Vaccine (Definition)

Reaction to Hib vaccine: Mild reaction to shot includes local redness and fever. Usually occurs within 3 days of having injection. Major reaction: Such reactions from a vaccine are very rare, estimated at fewer than 1 in a million doses, and would happen within a few minutes to a few hours after the vaccination. Major reaction include hives, swelling of the face and throat, difficulty breathing, a fast heartbeat, dizziness, and weakness. These would usually start a few minutes to a few ...Read more


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What exactly a polymerase chain reaction is or a "pcr test?

Nucleic acid testing: Pcr allows enormous expansion of a chosen, limited segment of dna and facilitate testing of nucelic acid abnormalties. Pcr by itself is not a test. The specificity of the test is detemined by the composition of the probes used to analyse the nucelic acids. ...Read more

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How many types of pcr(polymerase chain reaction) is available?

Probably one.: In the rt-pcr test, viral RNA is extracted from the patient's plasma and treated with reverse transcriptase (rt) to convert the viral RNA into cdna. The polymerase chain reaction (pcr) process is then applied. This is then quantified. There is a quantiplex bdna or branched dna test, but it is done by centrifuge to concentrate and detect by a color reaction that can be quantified, not pcr. ...Read more

Dr. R Smith Dr. Smith
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What is the definition or description of: polymerase chain reaction?

Dr. R Smith Dr. Smith
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PCR: Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other dna tests (eg paternity). ...Read more

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How could PCR (polymerase chain reaction) be used to diagnose diseases?

How could PCR (polymerase chain reaction) be used to diagnose diseases?

Complicated issue: Pcr facilitates investigation of nucleic acid (dna and rna)abnromaltiies and some of the abnormalties are associated with specific diseases. ...Read more

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By explaining what exactly a polymerase chain reaction is or a "pcr test?

DNA amplification: Poymerase chanin reaction/pcr is a process to amplify a specific stretch of dna. Pcr is a way to perform a number of tests by using specific probes. Pcr by itself is not a test, but one step in many tests. ...Read more

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What is the polymerase chain reaction and what is its purpose in medicine?

What is the polymerase chain reaction and what is its purpose in medicine?

PCR: This is a landmark advance in DNA testing of various samples, allowing very small sample to be amplified to determine if specific segments of DNA code are present. It is used extensively now in routine microbiology, and in other settings as well in the clinical lab. Some oncology applications also exist. ...Read more

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What is polymerase chain reaction; how does it find little pieces of dna?

Maked little pieces: Polymerase chain reaction is a process for making copies of small segments of dna. The target segment is identified by probes that are complementary in structure to the native dna. The gap between the probe targets is filled in my the polymerase enzyme and the process is repeated multiple time with exponential increase in the number of fragments generated. ...Read more

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How could polymerase chain reaction detect bacteria and viruses?

How could polymerase chain reaction detect bacteria and viruses?

Specific DNA probes: Each organism has unique DNA and or RNA and probes specific to each organism can be created. Such probes allow the organisms to be detected in biological or other samples. For an example of such a test, visit this site: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC265465/ ...Read more

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I just had polymerase chain reaction (pcr) HIV test negative at 4 weeks 6 days, almost 5 weeks?

I just had polymerase chain reaction (pcr) HIV test negative at 4 weeks 6 days, almost 5 weeks?

Probably free: There are rare cases in which antibody testing has remained negative for several months, but you probably do not have hiv. If you are in a state of anxiety over this and can afford it, a PCR for the presence of viral RNA in your blood will solve this definitively. ...Read more

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I recently did a HIV test(polymerase chain reaction), 3days post PEP, results were negative. Results paper is stating that it's recommended that one does a such a test two weeks after discontinuity of PEP. Are my results accurate or not?

I recently did a HIV test(polymerase chain reaction), 3days post PEP,  results were negative. Results paper is stating that it's recommended that one does a such a test two weeks after discontinuity of PEP. Are my results accurate or not?

Cant say: If really want to be sure you will(irrespective of PCR test)have to do a 4th generation Eliza at 1 and 3 months post exposure,followed by an HIV nucleic acid test at 6 months.PEP can interfere with PCR results if done too soon after PEP
Wish you well ...Read more

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Cutaneous B cell lymphoma Polymerase Chain Reaction test came inconclusive. One red lesion on scalp 28 yr old. Are chances for skin lymphoma small?

Cutaneous B cell lymphoma Polymerase Chain Reaction test came inconclusive. One red lesion on scalp 28 yr old. Are chances for skin lymphoma small?

Cutan BCL-PCR?: JulietaS~ why was this test done? For 1 small red lesion on the scalp? And why was THAT disease specified?If this was done without specific need to exclude THAT specific diagnosis, it makes no sense whatsoever! Please go and see a Dermatologist for this to be sorted out but in general, doing these highly specific tests to exclude a diagnosis in a very non-specific way is irresponsible. Thanx ...Read more

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Is PCR should be repeated if it gave negative result after exposure by one month, if yes after how much time shd be repeated ?

Did you take : Prophylaxis? It would be important to test six months post-exposure. You may do antibody tests periodically rather than repeating pcr tests. A test has been approved for home use. ...Read more

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After how many days wil PCR give perfect result in hiv?

After how many days wil PCR give perfect result in hiv?

What is perfect : Result? A pcr test for HIV can be done in less than one day. ...Read more

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Can HIV take 2 or more year to be detected by 4th gen or PCR tests? When the testing after a last exposure give 100% conclusive result? 2,3,5 years?

Can HIV take 2 or more year to be detected by 4th gen or PCR tests? When the testing after a last exposure give 100% conclusive result? 2,3,5 years?

You're good, Sam: The new tests are altogether trustworthy a month after the last risky event. Be thankful for good health, love wisely and well from now on, and treasure the memories of having shared love even it was not perfect. Best wishes. ...Read more

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Got negative result at 15 th day of my post exposure. Now can I trust on the HIV proviral DNA PCR result?

May be too early: Hiv nucleic acid test may not turn positive in two weeks. A negative result is good but you need to be retested in three too six months to be sure. Did you get post-exposure prophylaxis? Please talk to your doctor about prophylaxis, informing your sex partners and your follow-up care. ...Read more

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Is an Hiv Pcr test result reliable if taken 10 days and 20 hours (almost 11)after the exposure?? Thank you

Very good, not 100%: Around 95% of newly infected people have positive PCR tests by 10-11 days. So your result is very reassuring, but for conclusive testing also have an antibody test (or duo test, i.e. antibody + p24 antigen) at 4 weeks. In the meantime, you can relax, especially if your exposure was not particularly high risk, e.g. unprotected anal sex with an infected or high risk male partner. ...Read more

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Are HIV RNA and HIV PCR the same tests? How soon after potential exposure can you get a conclusive result?

HIV viral load tests: The short answer is 'yes' -since HIV-1 is an RNA virus. We quantify how much virus is there per drop of blood by using PCR technology (polymerase chain reaction if you must know). These days, the tests are extremely good -so after an exposure, this kind of testing could pick up positive results within 2 or 3 weeks. If you think you've been exposed, see an ID doc immediately-they could help w/ meds ...Read more

Past Reaction To Flu Vaccine (Definition)

No vaccine is 100% safe. Allergic and serious reactions are possible. In 2 cases, the early oral live polio vaccine and the first rotavirus vaccine, problems did occur, and some were serious. It is important to note, however, that even in these cases, the vaccines were withdrawn and the severe events were still far fewer than the number of lives saved. Both of these vaccines have been changed ...Read more


Polymerase Chain Reaction (Definition)

Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other ...Read more