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Doctor insights on: Polymérase Chain Reaction

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How could polymerase chain reaction detect bacteria and viruses?

How could polymerase chain reaction detect bacteria and viruses?

Specific DNA probes: Each organism has unique DNA and or RNA and probes specific to each organism can be created. Such probes allow the organisms to be detected in biological or other samples. For an example of such a test, visit this site: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC265465/ ...Read more

Polymerase Chain Reaction (Definition)

Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other ...Read more


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Can a monclonal IgM kappa light chain gammopathy turn into macrogobulinemia-waldenstrom?

Can a monclonal IgM kappa light chain gammopathy turn into macrogobulinemia-waldenstrom?

Yes it is possible: It would be one of differntial diagnosis. You need a bone marrow biopsy basically to check if this is caused by plasma cells or lymphplasmacytic cells etc. ...Read more

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Can a IgM kappa light chain monoclonal gammopathy turn into macroglobulinemia?

Can a IgM kappa light chain monoclonal gammopathy turn into macroglobulinemia?

IgM is macroglobulin: Macroglobulinemia is the term for monoclonal igm. However what you described is confusing/erroneous term, igm kappa is not light chain gammopathy. Igm kappa, by definition is macroglobulin. ...Read more

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How could PCR (polymerase chain reaction) be used to diagnose diseases?

How could PCR (polymerase chain reaction) be used to diagnose diseases?

Complicated issue: Pcr facilitates investigation of nucleic acid (dna and rna)abnromaltiies and some of the abnormalties are associated with specific diseases. ...Read more

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By explaining what exactly a polymerase chain reaction is or a "pcr test?

By explaining what exactly a polymerase chain reaction is or a "pcr test?

DNA amplification: Poymerase chanin reaction/pcr is a process to amplify a specific stretch of dna. Pcr is a way to perform a number of tests by using specific probes. Pcr by itself is not a test, but one step in many tests. ...Read more

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How many types of pcr(polymerase chain reaction) is available?

Probably one.: In the rt-pcr test, viral RNA is extracted from the patient's plasma and treated with reverse transcriptase (rt) to convert the viral RNA into cdna. The polymerase chain reaction (pcr) process is then applied. This is then quantified. There is a quantiplex bdna or branched dna test, but it is done by centrifuge to concentrate and detect by a color reaction that can be quantified, not pcr. ...Read more

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What is an anaphylactic type 1 hypersensitivity reaction?

What is an anaphylactic type 1 hypersensitivity reaction?

IgE mediated: Type 1 hypersensitivity is an immediate, ige-mediated reaction to a trigger (food, insect sting, etc.). ...Read more

Dr. Liawaty Ho Dr. Ho
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Can monoclonal IgM kappa light chain gammopathy turn into multiple myeloma?

Dr. Liawaty Ho Dr. Ho
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Can monoclonal IgM kappa light chain gammopathy turn into multiple myeloma?

It can: Yes, it can . It will depend on how much is the quantity of the monoclonal proteine, how many plasma cells on the bone marrow and ultimately whether one has end organ damages/ whether some other organs are affected significantly by the monoclonal protein. That is why some blood work, imaging study and bone marrow biopsy need to be done. ...Read more

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Can you interpret, squam epi cells few a, alpha 2 protein 1.01 h, possible faint IgM lambda monoclonal protein present, ACE serum 75 h?

Can you interpret, squam epi cells few a, alpha 2 protein 1.01 h, possible faint IgM lambda monoclonal protein present, ACE serum 75 h?

No: Can't interpret random blood test results without context. Talk to the doctor that ordered the tests. Most medical tests (x-rays, blood tests, etc., ) are better at telling us what you don't have rather than diagnosing what (if anything) you do have wrong with you. ...Read more

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What is a result of anaphylaxis(a systemic allergic reaction)?

What is a result of anaphylaxis(a systemic allergic reaction)?

Many causes: Severe allergic reactions (anaphylaxis) can be from stinging insects, foods, medications, latex, allergy shots, exercise, and idiopathic (about 1/3 of reactions, there is no cause found). These reactions can resolve with treatment, but some go on to be fatal especially if there is a delay in giving Epinephrine or the person has asthma. ...Read more

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What does "mast cell degranulation" mean in an allergic reaction?

What does "mast cell degranulation" mean in an allergic reaction?

Mast cell: Definition protein involved in mast cell degranulation. Mast cell is a large, ovoid cell of hematopoietic lineage, with a centrally located nucleus and numerous large, intensely basophilic granules. The binding of an antigen to its specific immunoglobulin-e antibody on the mast cell surface triggers the release of the mc granules. Mast cells are involved in hypersensitivity reactions. ...Read more

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Does a monoclonal antibody reactive with the o139 antigen of e.Coli would cross react with v.Cholera o139?

Does a monoclonal antibody reactive with the o139 antigen of e.Coli would cross react with v.Cholera o139?

Apparently not.: This does not seem likely because first that the v cholera is usually found in india and bangladesh while ecoli is usually in the U.S., mexico, and south america. Second, the mechanisms are different because the genes for toxicity on the e coli strains are not present in the v cholera. Third, there seems to be no information on any cross reaction of these two o 139 strains. ...Read more

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Hi Doctor, I did blood testing of Liver. the result are Anitibody anti HBe or Aniticorp anti HBe : Reaction Positive & Antigene HBe Reaction Negative.

Hi Doctor, I did blood testing of Liver. the result are Anitibody anti HBe or Aniticorp anti HBe : Reaction Positive & Antigene HBe Reaction Negative.

Hepatitis B tests: An antibody means that your body reacted to something and tried to fight it off. That's your immune system doing what it's supposed to. An antigen is something like a piece of virus that shouldn't be there. Your test report shows you were exposed to Hepatitis B but luckily your body fought it off. This HepBe test helps prove if you developed immunity from natural infection or from the vaccine. ...Read more

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Help please! is epipen (epinephrine) jr auto injectors used for all allergic reactions or just emergency reactions?

Help please! is epipen (epinephrine) jr auto injectors used for all allergic reactions or just emergency reactions?

Limited use: The value of an Epipen (epinephrine) is its ability to help block a tragic evolving allergic reaction and provide time for a person to reach urgent followup care.It's effect is relatively short (hour or so).If you simply break out in hives as a food reaction, it could help some, but since the food will be processed in your body for days, a longer agent like Diphenhydramine or citirazine would be more benificial. ...Read more

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High KAPPA/LAMBDA LIGHT CHAIN/slightly high ratio.No Monoclonal protein immunofixation or serum electrophoresis. Is this a type of cancer?Normal WBC

High KAPPA/LAMBDA LIGHT CHAIN/slightly high ratio.No Monoclonal protein immunofixation or serum electrophoresis. Is this a type of cancer?Normal WBC

I bet it's nothing: Without free light chains or a monoclonal protein in blood or urine, the ratio's meaningless. And like most lab tests, the reference range is set so that a few percent of healthies fall outside on either end. ...Read more

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What is an IgG lambda paraproteins?

Released immune cell: It is a single band shown on a special test of aprotein plasma cells or white cells called lymphocytes. It sometimes causes symtpoms and is usually nothing to worry about. But i presume your dr. Did this..So speak to him/her about whether any other tests are needed..Or followup with a specialist. ...Read more

Dr. R Smith Dr. Smith
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What is the definition or description of: polymerase chain reaction?

Dr. R Smith Dr. Smith
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PCR: Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other dna tests (eg paternity). ...Read more

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Can juvederm reaction cause an allergic reaction?

Can juvederm reaction cause an allergic reaction?

Yes: It would be an extremely rare instance since the main component is hyaluronic acid which is present as a protein in your cells. However there is a rare instance that you are allergic. There is no allergy testing required for HA drugs ...Read more

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Pcr (Definition)

Polymerase chain reaction is used in molecular biology to amplify copies of dna. It is used in dna cloning for sequencing, functional analysis of genes, identification of genetic finger prints, diagnosis of hereditary ...Read more