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Doctor insights on: Plate Count Agar

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What are the nutrients are in nutrient agar?

What are the nutrients are in nutrient agar?

Sugar and Beef: Sugar and beef extract (bouillon). The sugar provides the energy via glucose and the beef provides protein. Gelatin is used to solidify the agar. ...Read more

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Cled medium preferred over mcconkey agar in UTI because?

Cled medium preferred over mcconkey agar in UTI because?

Bacteriology: Cled medium helps to prevent swarming of proteus over the surface of the plate. When this occurs it can overgrow and prevent identification of other organisms which may have been present. Am unaware that there is universal acceptance that this is preferred over mcconkey, and am also not familiar with the frequency with which proteus creates a problem. ...Read more

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Why we do not use jelly instead of agar as a medium for the growth of bacteria?

Why we do not use jelly instead of agar as a medium for the growth of bacteria?

Jelly - solid or liq: Microbiologists want their bacteria to be on a well-defined, predictable, relatively pure media, so that everyone agrees on the resulting growth pattern, shape, color, etc. Preference is given to solid media (agar plates) or liquid media (broth). Therefore, new media, especially variable in components and textures, are rarely used. ...Read more

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Why mycobacteria tuberculosis cannot be cultured on blood agar?

Why mycobacteria tuberculosis cannot be cultured on blood agar?

TB culture: Tb is difficult to culture and culture results often take months. Lowenstin-jensen and middlebrook agars are the culture medias used to grow tb. ...Read more

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How could clostridium botulinum be cultured on agar?

How could clostridium botulinum be cultured on agar?

Huh?: You don't want to do that, otherwise you will have federal authorities knocking on your front door with a warrant for your arrest. ...Read more

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Does TB cause hemolysis of blood agar? And what of hemolysis?

Does TB cause hemolysis of blood agar? And what of hemolysis?

Question unclear: If you are asking a microbiology lab question about whether mycobacterium tuberculosis growth produces hemolysis, it is not generally grown on blood agar, generally does not produce hemolysins. It is generally cultured on specialized media and the type of hemolysis is not an issue in its identification. ...Read more

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Can you tell me in an immunodiffusion test to diagnose histoplasmosis, patient's serum is placed in a well in an agar plate?

Can you tell me in an immunodiffusion test to diagnose histoplasmosis, patient's serum is placed in a well in an agar plate?

Yes, it is: Immunodiffusion is a test where the antigen is placed in the center of the agar plate and the antibodies (Patient's sera that contain antibodies) in wells at the sides of the dish. The antigen and antibodies diffuse from the wells towards each other; where they meet, there forms a band (precipitate) that can be see. No band - negaative. ...Read more