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Doctor insights on: Pcr Test For Hiv

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What is the window period for p42 antigen/ antibody dual 4th gen HIV test? And the window period for HIV RNA PCR test? When can it be conclusive?

What is the window period for p42 antigen/ antibody dual 4th gen HIV test? And the window period for HIV RNA PCR test? When can it be conclusive?

See below: Please consult this site for a detailed answer. Nucleic acid tests are conclusive about a month after acquiring infection. http://www.sfaf.org/hiv-info/testing/hiv-test-window-periods.html ...Read more

Polymerase Chain Reaction (Definition)

Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other ...Read more


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I had unprotected oral sex on me I saw no blood I have no sores I am circumsized I have tooken a HIV dna pcr test at 18 days and a 46 also a 4 week and 6 week duo test and a 8 week rapid test am I ok?

I had unprotected oral sex  on me I saw no blood I have no sores I am circumsized I have tooken a HIV dna pcr test at 18 days and a 46 also a 4 week and 6 week duo test and a 8 week rapid test am I ok?

You're OK: Oral sex is safe sex for HIV, with no proved transmissions by fellatio from mouth to penis, only rare ones penis to oral. You didn't need testing on account of this event and for sure not the multiple expensive ones you had. But the results from that combination of tests are conclusive. For sure you didn't catch HIV during that exposure and no further testing is needed. Best wishes and stay safe! ...Read more

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How reliable is the PCR test for hiv?

How reliable is the PCR test for hiv?

Serology better: The pcr (viral load) is generally not recommended as a diagnostic test; it's used in people who are already known to have HIV to monitor therapy. The HIV serology (antibody test) is preferred for diagnosis, as pcr is more subject to false positives and false negatives. However, pcr can be used to diagnose acute retroviral syndrome (primary infection). It should be very high at that stage. ...Read more

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How soon can someone get a PCR test for HIV done after an exposure?

How soon can someone get a PCR test for HIV done after an exposure?

About 2 wks-3mos.: Antibodies to HIV appear as early as 2 weeks after infection, and seldom take longer than 3 months. Pcr can be done in days or weeeks after exposure, but is usually done after elisa and western blot since it is expensive and requires technical skill. ...Read more

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How accurate is PCR test for HIV after the 1st and 2nd week of exposure?

How accurate is PCR test for HIV after the 1st and 2nd week of exposure?

Likely to be neg: It takes about 25 days following exposure for the hin nat to become positive. If positive, it is nearly 100% accurate. Negative test does not exclude HIV in the body. ...Read more

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Is RNA PCR test for HIV accurate?

Is RNA PCR test for HIV accurate?

Yes: The nucleic acid test for HIV becomes positive about 20 days after exposure to the virus. ...Read more

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Aches 1year. 3hiv rapid test oraquick, unigold, clearview all negative after 6 months. 1st md said no more test. 2nd md said PCR test. More HIV test?

Aches 1year. 3hiv rapid test oraquick, unigold, clearview all negative after 6 months. 1st md said no more test. 2nd md said PCR test. More HIV test?

HIV is Hard to Get: You do not have hiv. Why in the heck would you keep testing? Why would you worry like you are. Aches can be signs of a million problems, not hiv. Aches are not in any way diagnostic for HIV infection. ...Read more

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Where can we (my boyfriend and i) get PCR test for HIV infection?

Where can we (my boyfriend and i) get PCR test for HIV infection?

Quest labs, Labcorp: If you are paying cash any private lab can perform the test. However they will charge far more than they charge the insurance companies so dmand a cash discount to the price they charge medicare which will be the lowest price. Probably still around $200. Insurance companies insist on the much less expensive antibody test. That test requires up to 6 months after last risky exposure to be reliable. ...Read more

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Does DNA PCR test for HIV 1&2 lose sensitivity over time? How accurate can a negative result for DNA PCR be on checking at 89 days post exposure?

Does DNA PCR test for HIV 1&2 lose sensitivity over time? How accurate can a negative result for DNA PCR be on checking at 89 days post exposure?

No sensitivity loss: These tests do not lose sensitivty over time. A negative result at almost 3 months is almost 100% conclusive. However, to reach 100% certainty, you also need an antibody test, if not already done. ...Read more

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Can i do PCR test for HIV, Hepatitis , HPV 18,16,12 after 32 days of risk exposure? The Result will be accurate and reliable? Peace of mind?

Can i do PCR test for HIV, Hepatitis , HPV 18,16,12 after 32 days of risk exposure? The Result will be accurate and reliable?  Peace of mind?

Yes and no: AT 32 days, you should not have a PCR test for HIV, only the standard antigen-antibody test (4th generation). It will be conclusive. Its too early for hepatitis, though; wait until 6 weeks (although hepatitis is so rare after any single exposure that most experts don't recommend testing at all). Don't waste money on HPV testing; it takes several months to show up, and everybody gets HPV anyway. ...Read more

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Is a PCR RNA test for HIV the same as a nucleic acid test? A doctor has told me this is no more accurate than a DUO test after 28 days post exposure..

Is a PCR RNA test for HIV the same as a nucleic acid test? A doctor has told me this is no more accurate than a DUO test after 28 days post exposure..

See below: PCR RNA for HIV and nucleic acid test are the same. Nucleic acids tests are both more sensitive and specific than antibody and antigen (DUO) tests. ...Read more

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I just had polymerase chain reaction (pcr) HIV test negative at 4 weeks 6 days, almost 5 weeks?

I just had polymerase chain reaction (pcr) HIV test negative at 4 weeks 6 days, almost 5 weeks?

Probably free: There are rare cases in which antibody testing has remained negative for several months, but you probably do not have hiv. If you are in a state of anxiety over this and can afford it, a PCR for the presence of viral RNA in your blood will solve this definitively. ...Read more

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How conclusiv is neg 3,8and 12 wks combo and PCR RNA test for HIV after unprot vag expo even wth symps like rashes, sorethroat, diarrhea? Pls ans, tks

How conclusiv is neg 3,8and 12 wks combo and PCR RNA test for HIV after unprot vag expo even wth symps like rashes, sorethroat, diarrhea? Pls ans, tks

Extremely: If the tests are all negative it would be extremely unlikely that you have HIV. Now you just need to be safe from here on out and look into what else might be causing your symptoms. Good luck. ...Read more

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How reliable is PCR HIV test 1 month after an exposure?

Not the best test: The better test 1 month after exposure is either a standard serology (elisa with confirmatory western blot if positive) or a 4th generation antibody/antigen test. Hiv PCR (viral load) will usually be positive, but false positives and negatives can occur, so it's an imperfect diagnostic test. Viral load is used diagnostically mainly in people with symptoms of acute infection. ...Read more

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How accurate is a PCR Hiv test taken 14days past Exposure?

How accurate is a PCR Hiv test taken 14days past Exposure?

Not altogether: If you receive a negative result, follow it up in a few more weeks. But I am glad you are getting tested and I wish you the best. Whatever happens, I hope you have a memory of love that you will cherish forever, and that from now on, you have an experience that has helped you grow as a man. ...Read more

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Is it effective to take the PCR HIV test 27 days after unprotected sex?

For diagnosis: A positive test will prove HIV infection but a negative one will not exclude one at this stage. ...Read more

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What is the likelihood of a false negative on a PCR RNA TMA HIV test at 15-20 days post PEP/post exposure? I have heard of false positives.

Too soon to rule: out HIV. The PCR RNA test if negative at about 3 months after exposure would rule out infection. A negative result before than may not be definitive. A positive result be definitive any time. ...Read more

Dr. James Krick
16 doctors shared insights

Hiv (Definition)

Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more


Dr. Lisa Oldson
370 doctors shared insights

Hiv Test (Definition)

There are several ways to detect the HIV virus. The first is by antibodies. This includes eia (enzyme immuno assay). The rapid HIV tests are these types. The second way detects proteins on the viral coat (western blot). This is a gel plate electrophoresis. Thirdly there is pcr (polymerase chain reaction). This detects and amplifies dna allowing a viral ...Read more