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Doctor insights on: Pcr Rna Testing Accuracy After 7 Days

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What is the accuracy of HIV testing (antibody and antigen) 6 weeks post PEP? I had all tests (Neg) including the pcr rna test at 22 days post pep.

What is the accuracy of HIV testing (antibody and antigen) 6 weeks post PEP? I had all tests (Neg) including the pcr rna test at 22 days post pep.

HIV testing & RNA: Read the CDC updated recommendations on HIV testing. The rationale for this test being available is that HIV RNA is detectable in plasma before antibodies. A safe approach is to be tested after the eclipse period after potential exposure when no RNA nor antibodies to HIV can be detected. The RNA test is effective around 10 days after infection but seroconversion to antibodies may take 3 months. ...Read more

Polymerase Chain Reaction (Definition)

Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other ...Read more


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Hiv Test- 85 & 121 days 4th Gen test and RT RNA PCR negtiv. 149 days HIV 1/O/2 Ab (3rd gen) negtiv. Is this conclusiv or do I need more testing? Ongoing symps - lymph nodes for 3 mons. Pls ans, thks

Hiv Test- 85 & 121 days 4th Gen test and RT RNA PCR negtiv. 149 days HIV 1/O/2 Ab (3rd gen) negtiv. Is this conclusiv or do I need more testing? Ongoing symps - lymph nodes for 3 mons. Pls ans, thks

Not for HIV: It would help to know your risk factors for acquiring HIV, but, regardless, absence if viral DNA after 4 months followed by absence of HIV antibody at 5 months means that we're 99.9% certain you haven't acquired the HIV virus. However, you need to follow up with your doctor about the swollen lymph nodes. ...Read more

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How reliable is negative HIV PCR RNA test around 12 weeks after unprotected exposure? Do I need more testing? Thanks in advance

How reliable is negative HIV PCR RNA test around 12 weeks after unprotected exposure? Do I need more testing? Thanks in advance

Good to go, Mike: Be thankful for good health. Whatever happened, I hope you'll cherish a memory of having given and received love. Like all human love, it was imperfect and dangerous. Perhaps you're smarter from the experience -- it happens to all of us. Be wise, and perhaps there'll be someone soon with whom you'll find lifetime true love. ...Read more

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Hi, had HIV-1 rna pcr @ 5 weeks & 5 months. And HIV ag/ab DUO at 1, 2, 4 & 6 months. Do I need further testing for HIV or do I need HIV-2 pcr or did the DUO rule it out?, or is it too rare to worry about in MI? I leave in Novi, MI. Thank you

Hi, had HIV-1 rna pcr @ 5 weeks & 5 months. And HIV ag/ab DUO at 1, 2, 4 & 6 months. Do I need further testing for HIV or do I need HIV-2 pcr or did the DUO rule it out?, or is it too rare to worry about in MI? I leave in Novi, MI. Thank you

HIV: Assuming what you have described is accurate, you do not have HIV infection and do not need further testing unless there have been subsequent exposures. Good luck and be careful out there. ...Read more

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Prednisone for 2 weeks can affect hiv rna pcr test and antibody test? So please tell which test to do for accuracy? Recently diagnosed with Bell's palsy

Prednisone for 2 weeks can affect hiv rna pcr test and antibody test? So please tell which test to do for accuracy? Recently diagnosed with Bell's palsy

No: Prednisone is the average dose will not affect any of the tests. Potentially massive doses (over 60 mg) taken for many months may marginally affect the tests but the difference will likely be unimportant. ...Read more

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Is swollen lymphnod, fever, sorethroat, thrush means hiv? Tested for HIV PCR RNA n antibdy 2 times in 105 day period. What is other reason? Plz help

Is swollen lymphnod, fever, sorethroat, thrush means hiv? Tested for HIV PCR RNA n antibdy 2 times in 105 day period. What is other reason? Plz help

Asked and answered: You gave been repeatedly told that your symptoms are completely, with 100% accuracy not HIV. If you don't believe the experts here why do you keep asking the same question? ...Read more

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2 wks post exp., truvada for 21 days, end 6wks. At 6&7 wks PCR rna & 4th G results negative. 10 & 11 wks negative 4th G and rapid. Could I be + still?

2 wks post exp., truvada for 21 days, end 6wks. At 6&7 wks PCR rna & 4th G results negative. 10 & 11 wks negative 4th G and rapid. Could I be + still?

Test at 6 months: At 6 months after exposure, get a HIV nucleic acid test, if negative, that would be definitive.
Wish you good health!
For good health - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex, if you have sex. ...Read more

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Took Truvada 3wks post exp. Took it only 21 days. At 7, 8, 10, 11 and 13 wks did 4th G (7, 10, 13), PCR RNA (8), rapid (11) all negative. Conclusive?

Nearly so.: To be on the safe side, get a HIV nucleic acid test at 6 months after exposure.
Wish you good health! - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex, if you have sex. ...Read more

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How far RNA real time PCR for HIV detection is reliable after 28 days of exposure?

How far RNA real time PCR for HIV detection is reliable after 28 days of exposure?

HIV: Real time pcr for HIV RNA negativity means you are negative unless the test was somehow faulty. ...Read more

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Hiv rna I&2 qualitative pcr test after 10 days post receiving rimming negative. Is it reassuring?

Hiv rna I&2 qualitative pcr test after 10 days post receiving rimming negative. Is it reassuring?

Low risk, too soon: To test. Rimming, even if performed by a person with HIV is a relatively low risk exposure. To be sure, get a HIV nucleic acid test about 6 months after exposure.
Wish you good health! - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex, if you have sex. ...Read more

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I had a HIV RNA PCR and 4th generation test at 26 days post exposure. Results were negative, I have symptoms, how accurate are these results? Worried

I had a HIV RNA PCR and 4th generation test at 26 days post exposure. Results were negative, I have symptoms, how accurate are these results? Worried

Accurate but...: While the tests you have taken are good, the are not fool-proof. The standard is to retest at 6 months. This gives your body time to form antibodies. Be careful. ...Read more

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If 1hiv PCR RNA Quant test negative at 40, 105 days, is that enough to understand person has not got hiv?, or need more test on 6 mon? And What tests?

Probably enough: The definitive test to show no HIV infection is a negative antibody test and that should be done a minimum of 6 weeks after intercourse but usually more like 3-6 months. However if a pcr test is negative at 1 month and 3 months that's is essentially conclusive that you are negative, but you should have the Elisa and western blot antibody tests to be sure. ...Read more

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Took hiv pcr/rna test at 19 days but they ran wrong test so had to go back at 26 days. Came back undetect. (labcorp). at 26 days could load've dropd?

No, but too soon to: Rule out HIV. To be sure you should repeat the test at 6 months after exposure.
Wish you good health! - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex, if you have sex. ...Read more

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Is 59 days HIV combo + PCR RNA test neg conclusive? Some docs say yes, some say no and need to be tested at 3, 6, 12 months. What is correct? Some org say 12, 13 lots of confusing info Please answer!

The 3, 6, 12 is old: Based on data that was purely antibody; even the CDC website screws that up.

"HIV testing may be concluded 4 months after exposure "

http://nccc. Ucsf. Edu/wp-content/uploads/2014/03/Updated_USPHS_Guidelines_Mgmt_Occupational_Exposures_HIV_Recommendations_PEP. Pdf

Thing is, nobody including me, believes you have ONLY ONE EXPOSURE lifetime. So, the real situation is to be tested about every 6-12 mo ...Read more

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Had PCR RNA test (28 days after - used condom) - Result Negative. Having temp 99.7 today (6th week start), cough, dry eyes Should there be a concern?

Had PCR RNA test (28 days after - used condom) - Result Negative. Having temp 99.7 today (6th week start), cough, dry eyes Should there be a concern?

Be glad: You should be concerned that you put yourself at risk at all, but I'm glad you used a condom. With today's techniques, you should consider yourself uninfected and continue to love wisely and safely. Best wishes. ...Read more

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I had a HIV PCR RNA test done 14 days after high risk exposure and symptoms the result came back negative is this a good sign?

Yes: Repeat it in a few more weeks as your physician advises. Love safely from now on. And cherish having shared love, even if it was not perfect and did put you at risk. ...Read more

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Had unprtected vaginal sex (insertive).Had some symptoms after10 days Tested after 10 and 18 days rna pcr, 26 days hiv duo negative. Is it conclusive?

Had unprtected vaginal sex (insertive).Had some symptoms after10 days Tested after 10 and 18 days rna pcr, 26 days hiv duo negative. Is it conclusive?

Too early to know: You could have taken post-exposure prophylaxis, however that window has passed. If the HIV nucleic acid test is negative at 12 weeks after exposure, that is more or less conclusive, provided you do not keep exposing yourself, ...Read more

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Enhanced chemiluminscence assay fr HIV (-ve) result conclusive after 14 months. Earlr immuno assay (-ve) at 17 days, RNA PCR (-ve) at 33 days. Pls adv?

Enhanced chemiluminscence assay fr HIV (-ve) result conclusive after 14 months. Earlr immuno assay (-ve) at 17 days, RNA PCR (-ve) at 33 days. Pls adv?

Negative HIV: I understand that you've had a series of negative labs for hiv. I've glad to hear this. Very rare people never develop the antibody, and if you are still very afraid, see if you can get another pcr and be done with this. From now on, think about barrier protection both for yourself and whoever you love -- it's gentlemanly & may enhance your enjoyment in other ways. Good luck. ...Read more

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Ultra sensitive RNA PCR HIV 48-10, 000, 000 not dected along with 2nd gen antibody neg also 32days gonna retest with home access in5 days should I worry?

Ultra sensitive RNA PCR HIV 48-10, 000, 000 not dected along with 2nd gen antibody neg also 32days gonna retest with home access in5 days should I worry?

Probably not: Would retest in 6 months, but the absence of measurable HIV RNA makes this highly improbable that you are infected. The better question is how and why were you exposed, and do you plan precautions in the future. You may not believe this, but people lie about their sexual activities and whether or not they have std's. Be a boy scout and be prepared! ...Read more

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How accurate/sensitive is the HIV PCR, RNA test after 11days post exposure? Most clinics are saying over 99% accurate after 10 days but I have also read other percentages elsewhere.

VERY accurate: The HIV PCR RNA tests are more expensive but are accurate within a shorter time frame than antibody based tests. They are certainly very accurate. I'm sure you could get different experts to argue that they are 95% accurate vs 99% accurate. If you have a negative HIV PCR RNA test you should be reassured and not allow yourself to continue to feel anxious about it. Focus on not repeating situation ...Read more

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What is the likelihood of a false negative on a PCR RNA TMA HIV test at 15-20 days post PEP/post exposure? I have heard of false positives.

Too soon to rule: Out HIV. The PCR RNA test if negative at about 3 months after exposure would rule out infection. A negative result before than may not be definitive. A positive result be definitive any time. ...Read more

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UK clinics state that PCR RNA tests for HIV should be followed by a DUO test at 28 days post exposure as duo is more reliable after 28 days. Opinions?

CDC guidelines: For CDC guidelines regarding testing for HIV see the site below.
Nucleic acid testing for HIV is more sensitive and specific.

http://www. Aidsmeds. Com/articles/CDC_testing_protocol_1667_25832.shtml ...Read more

Pcr (Definition)

Polymerase chain reaction is used in molecular biology to amplify copies of dna. It is used in dna cloning for sequencing, functional analysis of genes, identification of genetic finger prints, diagnosis of hereditary ...Read more


Rna (Definition)

This is a building block for dna. Rna is what makes up the double helix and ...Read more