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Doctor insights on: Microparticle Enzyme Immunoassay

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Substrate name for lipase enzyme?

Substrate name for lipase enzyme?

Olive oil: At least that's what we used for assays when i was doing a lot of clinical chemistry. Today kitmakers sell you "lipase substrate" which the physician need only do quality control on. I suspect it's olive oil. ...Read more

Enzyme (Definition)

An enzyme causes the transformation of a compoond to another compound. In living structures, it is the transformation of a protein to another form. An example is from an inactive state ...Read more


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Apart from elisa, which other immunoassay(s) can quantitative antigen analysis use?

Apart from elisa, which other immunoassay(s) can quantitative antigen analysis use?

See below: Rocket electrophoresis, radial immunodiffusion, conventional radio-immunoassy, densitometric scanning. There are many variations of ELISA itself. ...Read more

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What are these tests done for? Hb, tc, ast, alt, bilurubin total, direct. Creatinine, quantitative CRP assay and rbs.

What are these tests done for?
Hb, tc, ast, alt, bilurubin total, direct. Creatinine, quantitative CRP assay and rbs.

General health: Just as a grown man, i might walk into my physician's office after several years of neglecting my annual physicals, and expect to get all of these checked. Health maintenance, fishing expedition, or focused workup -- all are common tests. ...Read more

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Immunofixation, Serum FAH = Monoclonal IgG kappa immunoglobulin. Beta FAH = 10.5% Abnormal band, immunofixation to follow. I'm IgA deficient too. ??

Immunofixation, Serum FAH = Monoclonal IgG kappa immunoglobulin.   Beta FAH = 10.5% Abnormal band, immunofixation to follow. I'm IgA deficient too. ??

Possibly: If I am understaning this right you have a monoclonal protien band? The most important next steps here would be to identify the band (immunofixation) and then perfrom quantitvaive immunoglobulin levels. IF there is a monoclonal band, you may very well have reciprocal depriciation of alternative immunoglobulins and IgA deficiecny with an IgG monoclonal protein is possible. ...Read more

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How to measure anti-dna antibody using farr assay?

How to measure anti-dna antibody using farr assay?

Radioactivity: The farr assay is a radioimmunologic assay. It uses radiolabeled antigens to detect specific antibodies in serum. The antigens are allowed to react with the serum and then precipitated using a special reagent such as protein a sepharose beads. The bound radiolabeled immunoprecipitiate is commonly analzyed by gel electrophoresis. ...Read more

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-incom. Rt. Bundle branch block -cholesterol (total), serum by enzymatic method 208 -biochemistry lipid profile : LDL cholesterol, serum 133 ?

-incom. Rt. Bundle branch block 
-cholesterol (total), serum by enzymatic method 208
-biochemistry lipid profile : LDL cholesterol, serum 133

?

Unlikely 2B related: Total and LDL cholesterol look ok, unless you have heart disease or multiple risk factors for heart disease, then LDL goal is <100. Inocmplete rbbb can be normal. Sometimes is just actually a "r s r-prime" pattern in certain electrocardiogram leads- many times a normal finding seen in healthy young military recruits. Incomplete rbbb can progress to a complete right bundle block. ...Read more

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Serum -tiny IgM kappa monoclonal protein b2 region, urine - tiny IgM k paraproteinemia in gamma region. Was a mistake made or is lab normal?

Serum -tiny IgM kappa monoclonal protein b2 region, urine - tiny IgM k paraproteinemia in gamma region. Was a mistake made or is lab normal?

Monoclonal: multiple myeloma (g>a>m>e, d) •primary macroglobulinemia (waldenstrøm’s, igm) •monoclonal gammopathy of undetermined significance (mgus) (converts at 2% per year to myeloma) •miscellaneous conditions •(converts at 2% per year to myeloma) these all have to be thought of by the hematologist. ...Read more

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LDH Isoenzymes lab test results: LDH total- 153. LD1- 32, LD2-30, LD3, 20, LD4- 8, LD5- 10. Are these results normal? Is the LD1&LD2 ratio abnormal?

LDH Isoenzymes lab test results: 
LDH total- 153. LD1- 32, LD2-30, LD3, 20, LD4- 8, LD5- 10. Are these results normal? Is the LD1&LD2 ratio abnormal?

Historic test: Matthew, the LDH isoenzyme test is ancient history, almost never ordered nowadays. And your LDH is normal. The old 1-2 ratio is an antique screen for a heart attack, and it's neither sensitive nor specific. I know you're obsessing over the idea that you have lymphoma, and a few websites suggest this as a screening test -- but it's not. Focus on building a life for yourself. ...Read more

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Increased aldolase, complement c3/c4 serum, sed rate, c-react. Protein, beta globulin, monocytes, bun/creatin low mcv-mch, vit d-possible causes?

Increased aldolase,  complement c3/c4 serum, sed rate, c-react. Protein, beta globulin, monocytes, bun/creatin low mcv-mch, vit d-possible causes?

Complicated: When i was doing clinical medicine, these were collectively sort of the flags for sarcoid disease but I am not saying that's the case here. This is because sarcoid is a diagnosis of exclusion so the advice is to rule out other diseases with additional diagnostic tests which can be done by a primary care physician or rheumatologist. ...Read more

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Is chemiluminescence Immunoassay a treponemal test?

Is chemiluminescence Immunoassay a treponemal test?

Not necessarily: Chemiluminescence is an indicator in many immuno- assays. It is not a test per se. ...Read more

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HSV 1 positive through blood test =[LIAISON assay using chemiluminescence immunoassay (CLIA) tech.] Index=1.47, how likely is it false positive?

HSV 1 positive through blood test =[LIAISON assay using chemiluminescence immunoassay (CLIA) tech.]  Index=1.47, how likely is it false positive?

Not false positive: False positive tests for HSV1 are very rare. Half of all persons your age have positive tests, so nothing unusual there. Most likely from an oral infection in the distant past. ...Read more

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Serum free light chain test shows normal kappa (8.7mg/dl), low lambda (3.80), high kap/lam ratio (2.31). Serum $ urine electrophoresis show no monoclonal protein, nor does serum immunofixation. Likely MGUS, myeloma, or admyloidosis? CBC/met panel ok.

Serum free light chain test shows normal kappa (8.7mg/dl), low lambda (3.80), high kap/lam ratio (2.31). Serum $ urine electrophoresis show no monoclonal protein, nor does serum immunofixation. Likely MGUS, myeloma, or admyloidosis? CBC/met panel ok.

False positive rate: There is 30% or so false positive rate of kappa/lambda ratio in people without MGUS, myeloma or other disorders. For good health - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form. Practice safe sex. ...Read more

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Thyroid Peroxidase Antibodies 274.0 Anti-Thyroglobulin Antibody 164.4 Mean Platelet Volume 9.1 Erythrocyte Sed Rate 26 Estimat Glomerular Filtratio 43?

Thyroid Peroxidase Antibodies 274.0
Anti-Thyroglobulin Antibody 164.4
Mean Platelet Volume 9.1
Erythrocyte Sed Rate 26
Estimat Glomerular Filtratio 43?

Thyroid and kidneys: Hi. You have antibody evidence of thyroid autoimmunity. It would be nice to know your TSH and free T4. Are you on thyroid hormone? You have diminished kidney function. Do you know why? ...Read more

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Is a IgG quantitative test same as the serum protein electrophoresis test?

Is a IgG quantitative test same as the serum protein electrophoresis test?

Blood tests: They are two different separate blood tests, spep serum protein electrophoresis test is a screening test for plasma cell disorders like mgus, smoldering or full blown myeloma and quantitative igg is a blood test to quantify the amount of igg in blood. Usually more than 3 grams is significant in blood. ...Read more

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High KAPPA/LAMBDA LIGHT CHAIN/slightly high ratio.No Monoclonal protein immunofixation or serum electrophoresis. Is this a type of cancer?Normal WBC

High KAPPA/LAMBDA LIGHT CHAIN/slightly high ratio.No Monoclonal protein immunofixation or serum electrophoresis. Is this a type of cancer?Normal WBC

I bet it's nothing: Without free light chains or a monoclonal protein in blood or urine, the ratio's meaningless. And like most lab tests, the reference range is set so that a few percent of healthies fall outside on either end. ...Read more

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Please interpret- C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

You have chlamydia: "Detected" means the test found chlamydia -- i.e. a positive result. Return to the doctor or clinic where the test was done for treatment. (If you tested yourself at a lab, see a doctor or clinic ASAP.) Your current or recent sex partner(s) also need treatment. Good luck! ...Read more

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Blood flow cytmtry shows dual positive cd4cd30 cells absolute count of 2354. IGG slightly elevated with elevated light chains, T cell Beta clone noted?

Blood flow cytmtry shows dual positive cd4cd30 cells absolute count of 2354. IGG slightly elevated with elevated light chains, T cell Beta clone noted?

Asking what?: If your are requesting a diagnosis online, this is not the appropriate venue. Though the flow cytometry results are not totally normal, your results should be reviewed with the physician who ordered it. This format does not give us the ability to determine if your clinical presentation and lab results are consistent with a specific diagnosis. ...Read more

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Electron transport chains and ATP synthase enzymes are embedded within what?

Electron transport chains and ATP synthase enzymes are embedded within what?

Mitochondria: It sounds like you have been reading about mitochondria, the powerhouse organelle of cells. These organelles use methods to create the cellular currency of energy, Adenosine triphosphate. You probably read that a portion of the ATP synthase complex is embedded in the mitochondrial membrane. Read about it here: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3278611/ You can consult me if you have Q's ...Read more