Top
20
Doctor insights on: Lyme Disease Dna R T Pcr Blood

Share

Polymerase Chain Reaction (Definition)

Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other ...Read more


2

2
Can you detect spirochete infections(leptospirosis, borreliosis, syphilis) via blood culture tests? If not, why?

Can you detect spirochete infections(leptospirosis, borreliosis, syphilis) via blood culture tests?  If not, why?

Spirochaetes: These organisms are exceptionally difficult, if not impossible, to grow in artificial media. They can be diagnosed by special stains, serologically by antibody formation, and in some instances by detection of antigens by nuclear protein detection. ...Read more

3

3
Hiv western blot- all antibodies -ve except 1p17 detected. Hiv tridot is negative.What is further advise?

Hiv western blot- all antibodies -ve except 1p17 detected. Hiv tridot is negative.What is further advise?

HIV: Hiv western blot confirmation is generally not necessary with some of the newer serological tests available. The best measure is a pcr for HIV rna. This is expensive, but definitive when negative. ...Read more

4

4
Can you explain what the following blood tests will show? anti ena id, dna ab ds+conf bld, ccp antibody igg, ana blood

Can you explain what the following blood tests will show? anti ena id, dna ab ds+conf bld, ccp antibody igg, ana blood

Complicated: The question is too involved to address in this forum. Please consult this site for an introduction. http://www.kidneyabc.com/special-tests/2.html ...Read more

5

5
Hep c anti body 14 with cutoff of 1, but qualitative HCV RNA PCR says no RNA material detected. In notes says single test not enough . My alt, cbc, ok

Hep c anti body 14 with cutoff of 1, but qualitative HCV RNA PCR says no RNA material detected. In notes says single test not enough . My alt, cbc, ok

Probably cleared: The hcv viral load tests are not fda approved to confirm active infection, although this is how most doctors use it. If a repeat viral load in around 4 months is also negative, people are considered to have spontaneously cleared infection. ...Read more

6

6
Can dawson fingers brain lesions present in lymes disease? Im hoping I have lymes and not ms. My igenex test was positive and ELISA test CDC neg.

Can dawson fingers brain lesions present in lymes disease? Im hoping I have lymes and not ms. My igenex test was positive and ELISA test CDC neg.

Possibly: Dawson's fingers which appear on imaging is more consistent with demyelinating disease such as multiple sclerosis and not infection such as lyme. You need to have a more detailed evaluation and may need additional studies to make a definitive diagnosis. ...Read more

See 1 more doctor answer
9

9
Hep B surface Ab - positive, Hep BE antigen - positive, Viral DNA 2000 IU. Acute Hep B DX. Tests don't match the serology timeline? Am I in recovery?

Hep B surface Ab - positive, Hep BE antigen - positive, Viral DNA 2000 IU. Acute Hep B DX. Tests don't match the serology timeline? Am I in recovery?

Need expert care: This situation is too complex for safe advice on this forum. You should be seeing an expert in person, or perhaps seek HealthTap's concierge service with an infectious diseases or liver disease expert. ...Read more

11

11
Help with blood results: EBV VCA IgG >8.0, EBV IgM neg. CMV antibody IgG 38, EBV NA antibody >8.0. Can it explain my body aches? Why or why not?

Help with blood results: EBV VCA IgG >8.0, EBV IgM neg. CMV antibody IgG 38, EBV NA antibody >8.0. Can it explain my body aches? Why or why not?

Old infection: with EBV is the pattern of IgG positive (including NA) and IgM negative. I assume the CMV which is a different infection is also the IgG? If so, both are old infections and do not usually explain your current symptoms. This is a pattern that shows immunity to these infections. Look for other causes. ...Read more

12

12
HCVantibody screen is reactive with a low signal to cutoff ratio.2 con.HCV RNA PCR tests 2 months apart are not detected or negative.Does one has hepC

No: PCR test is more reliable. For good health - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form. Drink enough water daily so that your urine is mostly colorless. Practice safe sex. ...Read more

13

13
Have IgG and IgM for CMV but PCR not detected. Pos rmsf IgM neg igg. Bands in CSF mri/white matter foci. Many symptoms possible ms.. No real answers?

Have IgG and IgM for CMV but PCR not detected. Pos rmsf IgM neg igg. Bands in CSF mri/white matter foci. Many symptoms possible ms.. No real answers?

Uncertainty: I am unclear as to what is really going on. Appears that you have immune activity in the central nervous system. You have some cross-reactivity in the serum for CMV and rickettsial infection. Don't think that need to add another disease to the picture. Best to discuss with your doctor or get a second opinion from another specialist. ...Read more

See 1 more doctor answer
14

14
Cmv IgG positive CMV IgM postive , all CMV infectious disease blood tests are postitive. Liver enzymes slight elevated , what do I have? Cmv is what

Cmv IgG positive  CMV IgM postive , all CMV infectious disease blood tests are postitive. Liver enzymes slight elevated , what do I have? Cmv  is what

CMV: Is cytomegalovirus and is similar to mono. It is causing the positive blood tests and the elevated liver enzymes. It is very common. You will recover. In the meantime, I do suggest seeing an acupuncturist and chinese medicine practitioner as it can really help your body recover faster. ...Read more

16

16
Cdc HIVtestingAlgorithmRecommendationFinal doc, pge11, suggest HIV antibody r antigen r viral load detect by day 50 for diag. then y some docs disagre

Cdc HIVtestingAlgorithmRecommendationFinal doc, pge11, suggest HIV antibody r antigen r viral load detect by day 50 for diag. then y some docs disagre

No test is perfect.: Lab tests have false positives and false negatives. The accuracy of a diagnostic lab test also depends on how good the lab is, and how the test sample was collected. If detecting HIV was easy there would be only one test. ...Read more

17

17
Blood tests showed high antibodies to: ana, ssdna, sm, rnp/sm, ssa(ro), ssb(la), scl-70, centromere. Is this likely lupus or a different automimmune?

Blood tests showed high  antibodies to: ana, ssdna, sm, rnp/sm, ssa(ro), ssb(la), scl-70, centromere.  Is this likely lupus or a different automimmune?

Prob false positive: To have all these antibodies to be positive is suggestive of a false positive result. Virus infection can cause similiar findings. Ultimately these results have to be interpretted in the context of your doctor's findings. Talk to your doctor. ...Read more

See 1 more doctor answer
18

18
What can cause false positive ELISA HIV test? 2 confirmatories neg and viral load neg. No bands detected.

What can cause false positive ELISA HIV test? 2 confirmatories neg and viral load neg. No bands detected.

Many things: An elisa test for HIV is very sensitive which basically means it is a good test for ruling out HIV and is only reliable when negative. A positive elisa test does not mean someone has HIV and is frequently called a "false positive" there are many causes of a false positive which include liver disease, blood tranfusions, infections, autoimmune disease and dumb luck! ...Read more

19

19
Hi drs.My blood test for CMV DNA assay results-not detected, what does this mean., ?I hve hepatitis b.

Hi drs.My blood test for CMV DNA assay results-not detected, what does this mean., ?I hve hepatitis b.

CMV: CMV is s common viral infection known as cytomegaly virus. Your blood test shows that you have not been exposed to this virus and thus are negative for cmv. ...Read more

Pcr (Definition)

Polymerase chain reaction is used in molecular biology to amplify copies of dna. It is used in dna cloning for sequencing, functional analysis of genes, identification of genetic finger prints, diagnosis of hereditary ...Read more


Dna (Definition)

Dna are the coding molecules found in all living things on earth which contain the instructions on how to build living things. Its is comprised the nucleotide molecules which are ...Read more