Top
20
Doctor insights on: Indirect Coombs Test Procedure

Share
Dr. Al Hegab Dr. Hegab
2 doctors agreed:
1

1
What are the differences between direct Coombs test and indirect Coombs test?

Dr. Al Hegab Dr. Hegab
2 doctors agreed:
What are the differences between direct Coombs test and indirect Coombs test?

Both test Ag-Ab rxns: Direct Coombs will test for the reaction using the patient’s RBCs, if there is a reaction, then the direct test is positive, used to test for autoimmune hemolytic anemia. Indirect Coombs test uses the patient’s serum (not rbcs) which is added to rbcs that are known to have a certain antigenicity, positive in cases of hemolytic diseases of the newborn, mother's serum against baby's RBCs, ...Read more

Coombs Test (Definition)

Coombs serum is an antibody directed against other antibodies that makes antibody-coated red cells stick together. The direct Coombs test seeks antibodies on a patient's own red cells that may be causing red cell damage and perhaps anemia. The indirect Coombs test is used in blood banking to determine what antigens are present on unknown red cells by seeing ...Read more


2

2
Blood test shows élévated bilirubin , urine test négative for bilirubin. Any explanation??

Blood test shows élévated bilirubin , urine test négative for bilirubin. Any explanation??

Unconjugated: Bilirubin exists in the blood in both the conjugated and Unconjugated forms. Only the conjugated can enter the urine. If you feel well, and this is new, you very likely have Gilbert's non-disease. Best wishes. ...Read more

3

3
HSV 1 positive through blood test =[LIAISON assay using chemiluminescence immunoassay (CLIA) tech.] Index=1.47, how likely is it false positive?

HSV 1 positive through blood test =[LIAISON assay using chemiluminescence immunoassay (CLIA) tech.]  Index=1.47, how likely is it false positive?

Not false positive: False positive tests for HSV1 are very rare. Half of all persons your age have positive tests, so nothing unusual there. Most likely from an oral infection in the distant past. ...Read more

See 1 more doctor answer
4

4
Please interpret- C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

Please interpret-
C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

You have chlamydia: "Detected" means the test found chlamydia -- i.e. a positive result. Return to the doctor or clinic where the test was done for treatment. (If you tested yourself at a lab, see a doctor or clinic ASAP.) Your current or recent sex partner(s) also need treatment. Good luck! ...Read more

5

5
Why can't you donate plasma with an abnormal indirect Coombs test result?

Why can't you donate plasma with an abnormal indirect Coombs test result?

Antibody to red cell: A person with positive indirect coombs test has antibodies to red cells. If such plasma is transfused into a person, s/he could suffer loss of her/his own red cells. ...Read more

6

6
Is a noninvasive prenatal paternity bloodtest, done with blood from alleged fathers, cfdna and 317.000 genetic markers a reliable test?

Is a noninvasive prenatal paternity bloodtest, done with blood from alleged fathers, cfdna and 317.000 genetic markers a reliable test?

Yes: Make sure the identity of the alleged fathers and pregnant mother are confirmed. Make sure no tampering with the blood. Science can reveal the dna. ...Read more

7

7
Is the latex agglutination CRP test done in lab?

Is the latex agglutination CRP test done in lab?

Yes,but mixing tests: A CRP is a lab test, but kits are avaialble for office use. The latex agglutination test measures rf in dilutional titers, but measurement is rarely done that way. ...Read more

8

8
What is the difference between the direct Coombs test and indirect Coombs test?

What is the difference between the direct Coombs test and indirect Coombs test?

Bound vs unbound ABs: Direct coombs' test is used to detect antibodies that are already bound to the surface of red blood cells. Many diseases and drugs (quinidine, methyldopa, and procainamide) can lead to production of these antibodies indirect coombs' test looks for unbound circulating antibodies against a series of standardized red blood cells. The indirect coombs' test is only rarely used to diagnose a condition. ...Read more

9

9
How's a poliovirus antibody level test performed?

How's a poliovirus antibody level test performed?

Neutralization assay: Poliovirus antibodies are tested via neutralization assay for polio type 1, polio type 2, and polio type 3 separately. Protection to one type of polio does not protect you from the others. One needs to have protective titers to all three types to be "immune". ...Read more

10

10
Pcr blood test for hsv1 & hsv2 both negative 7 weeks post exposure. Is this a reliable test vs igg antibody testing?

Pcr blood test for hsv1 & hsv2 both negative 7 weeks post exposure. Is this a reliable test vs igg antibody testing?

HSV testing: Both are good. A PCR blood test looks for circulating viral DNA. The IgG look for the development of an antibody against the virus. If these are not present 7 weeks after exposure then the most likely conclusion is that you did not acquire it. ...Read more

See 1 more doctor answer
11

11
Is Haptoglobin (mg/dl) test a blood chemistry test or hematological test?

Is Haptoglobin (mg/dl) test a blood chemistry test or hematological test?

Haptpglobin: Is a transport protein for the breakdown of hemoglobin. If is used to assess for RBC destruction. When the haptoglobin Is low there is an association with increased hemolysis ...Read more

14

14
Is a IgG quantitative test same as the serum protein electrophoresis test?

Blood tests: They are two different separate blood tests, spep serum protein electrophoresis test is a screening test for plasma cell disorders like mgus, smoldering or full blown myeloma and quantitative igg is a blood test to quantify the amount of igg in blood. Usually more than 3 grams is significant in blood. ...Read more

See 1 more doctor answer
15

15
I am diabetic..My lab test result show total bilirubin of 1.55 mg/dL direct bilirubin 0.44 and indirect bilirubin 1.11, other liver function test is n?

I am diabetic..My lab test result show total bilirubin of 1.55 mg/dL direct bilirubin 0.44 and indirect bilirubin 1.11, other liver function test is n?

Sometimes it is norm: Mild elevation of bilirubin in the presence of normal liver enzymes is not uncommon. It is an inborn mild defect which has no consequences. If you feel well, there is nothing further to be done. Note any previous Liver function tests and also watch it in future. Once, confirmed you can ignore it in the future. ...Read more

16

16
HSV 1+2 Serum test resut- 1.77 & HSV Type 1+2 PCR DNA result Negative. Can't understand the difference between two results.

HSV 1+2 Serum test resut- 1.77 & HSV Type 1+2 PCR DNA result Negative. Can't understand the difference between two results.

Need details: PCR will detect herpes virus if present on the skin. If no virus is shedding at the time, then it shows negative. But herpes is present in a dormant form in a nerve and is detected by noting serum antibodies. Antibodies are specific immunity which contain the virus but fail to reach inside a nerve. When the virus sometimes comes out onto the skin it can be detected by PCR. When the virus is only i ...Read more

17

17
Is the direct Coombs test done at birth testing the baby or the mother? Whose Coombs is being tested?

Is the direct Coombs test done at birth testing the baby or the mother? Whose Coombs is being tested?

Baby's Red cells and: mother's antibodies. The Direct Coombs test in a newborn is done to look for maternal antibodies that may have coated the baby's red cells. So, in a way both are being tested, but it is mainly to test for maternal antibody induced hemolytic anemia in the baby. ...Read more

18

18
Is an HIV ELISA test always done with an western blot test?

Is an HIV ELISA test always done with an western blot test?

No: In general, the elisa is done first, and the lab then does a western blot if the elisa is positive. However, it is possible to order just the western blot, in which case the elisa usually isn't done. ...Read more

19

19
What is the ssa/Ssb blot test antibody blood test?

What is the ssa/Ssb blot test antibody blood test?

A test: for a certain autoimmune condition. it's important to understand that these blood tests need to be interpreted carefully in the context of your symptoms and exam findings. a positive test doesn't necessarily mean you have the condition. ...Read more

20

20
Which specimen is require for direct fluorescent antibody and immunoassay test for detecting c.Trachcomatis?

Which specimen is require for direct fluorescent antibody and immunoassay test for detecting c.Trachcomatis?

Either: A sample of urethral specimen is needed and this depends on what lab is used. A urethral swab or urine is common. See your clinic and what is offered. ...Read more