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Doctor insights on: Immuno Radiometric Assay

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How to measure anti-dna antibody using farr assay?

How to measure anti-dna antibody using farr assay?

Radioactivity: The farr assay is a radioimmunologic assay. It uses radiolabeled antigens to detect specific antibodies in serum. The antigens are allowed to react with the serum and then precipitated using a special reagent such as protein a sepharose beads. The bound radiolabeled immunoprecipitiate is commonly analzyed by gel electrophoresis. ...Read more

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Immunofixation, Serum FAH = Monoclonal IgG kappa immunoglobulin. Beta FAH = 10.5% Abnormal band, immunofixation to follow. I'm IgA deficient too. ??

Immunofixation, Serum FAH = Monoclonal IgG kappa immunoglobulin.   Beta FAH = 10.5% Abnormal band, immunofixation to follow. I'm IgA deficient too. ??

Possibly: If I am understaning this right you have a monoclonal protien band? The most important next steps here would be to identify the band (immunofixation) and then perfrom quantitvaive immunoglobulin levels. IF there is a monoclonal band, you may very well have reciprocal depriciation of alternative immunoglobulins and IgA deficiecny with an IgG monoclonal protein is possible. ...Read more

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Please interpret- C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

Please interpret-
C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

You have chlamydia: "Detected" means the test found chlamydia -- i.e. a positive result. Return to the doctor or clinic where the test was done for treatment. (If you tested yourself at a lab, see a doctor or clinic ASAP.) Your current or recent sex partner(s) also need treatment. Good luck! ...Read more

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Hepatitis c (HCV) antibody is reactive. The hepatitis C Recombinant Immunoblat Assay(riba) confirmation test is non reactive.Is antibody false positiv

Hepatitis c (HCV) antibody is reactive. The hepatitis C Recombinant Immunoblat Assay(riba) confirmation test is non reactive.Is antibody false positiv

Well : A positive hepatitis C antibody test with a negative or in a test usually is not a false positive that usually means someone had hepatitis C infection and their body cleared the infection and they are now immune to hepatitis C. Do a Healthtap visit or see a primary care physician for specific diagnosis ...Read more

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Ebvab VCA IgM <36 (range 0-35.9), ebvearly antigenab IgG 37 (0-8.9), ebvab VCA IgG >600 (0-17.9), EBV nuclearantigenab IgG 409 (0-17.9) mono? No mono?

Ebvab VCA IgM <36 (range 0-35.9), ebvearly antigenab IgG 37 (0-8.9), ebvab VCA IgG >600 (0-17.9), EBV nuclearantigenab IgG 409 (0-17.9) mono? No mono?

You had mono...: Your mono-like symptoms likely prompted the testing- i hope you are starting to feel a little better. Testing for different antibodies (igm and igg) against ebv, requires careful interpretation. In early phases of infection, the igm level peaks first followed by a decline; levels of igg peak afterwards, and may remain elevated for quite a while. ...Read more

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HSV 1 positive through blood test =[LIAISON assay using chemiluminescence immunoassay (CLIA) tech.] Index=1.47, how likely is it false positive?

HSV 1 positive through blood test =[LIAISON assay using chemiluminescence immunoassay (CLIA) tech.]  Index=1.47, how likely is it false positive?

Not false positive: False positive tests for HSV1 are very rare. Half of all persons your age have positive tests, so nothing unusual there. Most likely from an oral infection in the distant past. ...Read more

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TSH with human anti-mouse antigen lab test correlation and purpose ?

TSH  with human anti-mouse antigen lab test correlation and purpose ?

Unclear questions: Mouse antibodies are used in many immunoassays, including test for TSH. Could you please restate your questions as it is not clear in its present form. You may visit this site for information on TSH. http://www.nlm.nih.gov/medlineplus/ency/article/003684.htm ...Read more

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Elevated EBV ab VCA IgG <8 and EBV nucleaer antigen ab IgG test 4.2. & anti-dna(ss)igg ab qn ( 119)& c react protien(5.6) what are implications?

Nothing.: Ebv antibodies indicate you had a previous infection, but not active. The c-reactive protein needs a units indicator - that is either normal or somewhat elevated. The anti ss dna is extremely nonspecific and very rarely indicated; I have no idea why that was ordered, discuss with your doctor. ...Read more

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Are HIV antibody tests accurate? Are hepatitis C antibody tests accurate?

Are HIV antibody tests accurate? Are hepatitis C antibody tests accurate?

Testing for Viruses: Yes. Of course. But, it does matter the timing of the tests and the length of time since the possible infectious exposure. ...Read more

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What is h.pylori blood antigen IGG and IGM meant ? IGG 17.95 and IGM 8.50

What is h.pylori blood antigen IGG and IGM meant ? IGG 17.95 and IGM 8.50

Not Diagnostic of: These tests do not indicate a recent infection.IGM has to be above. 40 and IGG is positive which suggest you might have been infected in the past. Only tests to confirm acute infection are stool for H Pylori Antigen and/ or Urea Bresth Test.Or CLOE test during endoscopy . Discuss your case with your Doctor or Gastroenterologist ...Read more

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Thyroid Peroxidase Antibodies 274.0 Anti-Thyroglobulin Antibody 164.4 Mean Platelet Volume 9.1 Erythrocyte Sed Rate 26 Estimat Glomerular Filtratio 43?

Thyroid Peroxidase Antibodies 274.0
Anti-Thyroglobulin Antibody 164.4
Mean Platelet Volume 9.1
Erythrocyte Sed Rate 26
Estimat Glomerular Filtratio 43?

Thyroid and kidneys: Hi. You have antibody evidence of thyroid autoimmunity. It would be nice to know your TSH and free T4. Are you on thyroid hormone? You have diminished kidney function. Do you know why? ...Read more

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Which specimen is require for direct fluorescent antibody and immunoassay test for detecting c.Trachcomatis?

Which specimen is require for direct fluorescent antibody and immunoassay test for detecting c.Trachcomatis?

Either: A sample of urethral specimen is needed and this depends on what lab is used. A urethral swab or urine is common. See your clinic and what is offered. ...Read more

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I have inconsistent tests that lean toward multiple myeloma or MGUS. First xray lytic lesion; globulin series serum & urine monoclonal positive to gamma; 24 hr electopherurine neg; beginning anemia; BUN off; other small abnormals. Bone survey neg.

I have inconsistent tests that lean toward multiple myeloma or MGUS. First xray lytic lesion; globulin series serum & urine monoclonal positive to gamma; 24 hr electopherurine neg; beginning anemia; BUN off; other small abnormals. Bone survey neg.

Need second opinion: The criteria for myeloma vs Mgus changed recently but both changes include exact percentages and size of lytic bone lesions. Your data as you summarized are not adequate and since mgus vs multiple myeloma are like dawn and day, you need to be seen and your precise numbers verified. That said, please see hemeonc in centers experienced in myeloma dx,management,and treatment. ...Read more

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Iga test results "insufficient". No levels recorded. Gliadin igiga an "not indicated". Tis.Transglut.Ab IgA "not indicated". Means?

Iga test results "insufficient". No levels recorded. Gliadin igiga an "not indicated". Tis.Transglut.Ab IgA "not indicated". Means?

Ok: If you have undetectable iga, any test involving IgA would be meaningless and negative regardless of the disease condition. You will need alternate testing and even intestinal biopsy to document your illness if gluten intolerance is suspected. Make sure to check the other classes of immunoglobulin to make sure you don't have an immune defect. ...Read more

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Which of these 3 hep c antibody screening tests(methodology)is the most accurate: Architect Anti-HCV, Advia Centauer HCV or Roche Eclia?

Which of these 3 hep c antibody screening  tests(methodology)is the most accurate: Architect Anti-HCV,  Advia Centauer HCV or Roche Eclia?

About equal: All three methods are FDA approved and about equally accurate. If you have concern about active Hep C infection, get a Hep C nucleic acid test. For good health - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form. Practice safe sex. ...Read more

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Apart from elisa, which other immunoassay(s) can quantitative antigen analysis use?

Apart from elisa, which other immunoassay(s) can quantitative antigen analysis use?

See below: Rocket electrophoresis, radial immunodiffusion, conventional radio-immunoassy, densitometric scanning. There are many variations of ELISA itself. ...Read more

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Who gets for testing CEA (carcinoembryonic antigen)?

Who gets for testing CEA (carcinoembryonic antigen)?

Certain cancers: Cea (carcinoembryonic antigen) can be found at elevated levels in the blood of patients with certain cancers such as colon, lung, breast. It is useful to follow if elevated at the time of diagnosis to correlate with response (if it goes down) to treatment or with relapse (if it becomes elevated again after having gone down to normal levels previously). It may be elevated in other benign conditions. ...Read more