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Doctor insights on: Human Karyotype Worksheet

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What is karyotyping?

What is karyotyping?

Chromosome study: A karyotype is a visual presentation of the chromosomes present in each cell, usually derived from blood lymphocytes. The cells are grown in tissue culture, then arrested in the stage of separation (mitosis), then dropped on a glass slide from about 2 feet, which breaks them open; a dye is added to make stain them, a photo taken, then arranged into their 23 pairs, then studied for anomalies. ...Read more

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How could the karyotyping results be explained ?

Need more info: Not sure of your question. Do you have a specific finding you would like explained? ...Read more

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What is the definition or description of: karyotyping?

See below: Karyotyping is a test to examine chromosomes in a sample of cells, which can help identify genetic problems as the cause of a disorder or disease. This test can:
•count the number of chromosomes
•look for structural changes in chromosomes

the test can be performed on almost any tissue, including:
•amniotic fluid
•blood
bone marrow
placenta. ...Read more

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What procedures are used in preparation for karyotyping?

One sample at a time: A blood sample is spun down and the white cells are separated into at least 3 tissue culture media sets. An amnio sample is spun down, the cells resuspended and divided into three tissue culture sets. Of primary importance: only one sample is ever set up at a time. No other sample is near another until the cultures have been completely set up and labeled. That prevents mixing up two babies by mistake ...Read more

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What happens if we assume that the vitiligo is generic disorder then can we check if it's a dominant, recesive and carrier etc. using karyotyping.?

What happens if we assume that the vitiligo is generic disorder then can we check if it's a dominant, recesive and carrier etc. using karyotyping.?

Autoimmune: Vitiligo is usually considered an autoimmune problem. If you want to find out whether it is genetic, you will need to get a detailed history on family members with this problem. I am not aware of any genetic markers uncovered thus far. Nonetheless if there has been no history of such a disorder among your immediate family members within 3 generations, genetic study would be meaningless. ...Read more

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Karyotyping - is it done to check all pregnant women over age 35years?

Not always: The decision to do an amniocentesis in order to do karyotyping is a personal decision and carries up to a 1 in 200 miscarriage rate. The information obtained would classically be used to decide upon termination or not as there is no treatable or correctable chromosome abnormality. At 35 years old and above, the risk of a chromosome defect is 1 in 200 or greater. ...Read more

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What's my chance to have normal baby if me or my husband have balanced translocation&we already had a ds baby? We didn't do karyotyping yet though

What's my chance to have normal baby if me or my husband have balanced translocation&we already had a ds baby? We didn't do karyotyping yet though

Genetic counselor: You need to have a karyotype done before you can determine your risk. It would be very important for you and your spouse to see a genetic counselor to be able to better understand your risk. ...Read more

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Can you request karyotyping during a routine physical/blood test?

Yes, but...: A karyotype is an expensive test - about $2000 dollars depending on what lab it is sent. Check with your insurance to see if they require pre authorization (ie approval for the diagnosis that your doctor is using the test for). If you have the test done without pre authorization on most plans, they would not cover it, and then the patient is responsible for the fees. ...Read more

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Why antibiotics are used in preparation media for karyotyping using giemsa banding?

Why antibiotics are used in preparation  media for karyotyping using giemsa banding?

Stop contaminatio: Because tissue culture is a relatively long and sensitive process, antibiotics are used in the media to prevent bacterial overgrowth from contaminating bacteria that could multiply, divide, and overwhelm the sensitive tissue culture. ...Read more

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Are there karyotyping like genetic level tests to confirm if a couple is compatible & will certainly have healthy children if they plan for pregnency?

Are there karyotyping like genetic level tests to confirm if a couple is compatible & will certainly have healthy children if they plan for pregnency?

Not really: There are no guarantees of compatibility or healthy children. The best thing to do is make sure that you and your partner are healthy and get early prenatal care if she becomes pregnant. If she intends to get pregnant, have her start taking a Folic Acid supplement (found in many vitamins) before she conceives. ...Read more

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For what reason antibiotics are used in preparation of media for karyotyping using giemsa banding?

For what reason antibiotics are used in preparation of media for karyotyping using giemsa banding?

Basically tissue : culture, antibiotics used to prevent bacterial contamination during the cell growth prior to the preparation of the karyotypes. ...Read more

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What's my chance to conceive with ds baby if me or my husband have the balanced translocation in karyotyping?

See geneticist: The best way to answer this question is to see a professional geneticist who can plot a family tree, consider your age, and calculate your risk based on your balanced translocation or your husband's. ...Read more

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My daughter was diagnosed with t cell all last year.Her karyotyping :abnormality in t(11;14)(p13;q11.2). Is there a targetted therapy for lmo2 issues?

My daughter was diagnosed with t cell all last year.Her karyotyping :abnormality in t(11;14)(p13;q11.2). Is there a targetted therapy for lmo2 issues?

Not yet: Gamma c gene inserted in scid patients; could cure the immune deficiency. But the two, gamma c gene was inserted close to lmo2 gene ( considered oncogene) which gets activated with gamma c gene insertion - causing increased proliferation on t cells ( causing t cell leukemia). Studies/ research on its way to target lmo2 gene. ...Read more

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Explain karyotyping test for muscular dystrophy?

Not exactly...: ..."karyotyping" - another kind of genetic testing is used:

http://www.Ncbi.Nlm.Nih.Gov/pmc/articles/pmc1715396/. ...Read more

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2 early MCs, heartbeat seen in 2nd case, no live birth, turner's synd in fetus 2nd case, karyotyping & antiphosopolipid normal. Way fwd? More tests?

2 early MCs, heartbeat seen in 2nd case, no live birth, turner's synd in fetus 2nd case, karyotyping & antiphosopolipid normal. Way fwd? More tests?

Try again: Abnormal chromosome patterns are seen in many of the MC's studied, so this would not be unexpected. If both you and spouse have been karyotyped & normal, you are not carrying a translocation that would doom you both to expect MC's.Consult the clinical genetics department at Toronto Children's if you have not already and see if their is anything more they recommend. ...Read more

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3 early MCs no live birth. 32yrs. All tests normal autoimmune, antiphosopolipid, hormonal, karyotyping. Turner's in 1 fetus. Should we try again & how?

3 early MCs no live birth. 32yrs. All tests normal autoimmune, antiphosopolipid, hormonal, karyotyping. Turner's in 1 fetus. Should we try again & how?

Yes: Don't give up, get followed by infertility/hi risk obstetrics doctor, at one point a medical geneticist input might be needed. For genetic / chromosomal disorders. PIGD, Pre- Implantation Genetic Diagnosis can be resorted to so as not to implant an embryo with a known major genetic/chromosomal disease, good luck ...Read more