Doctor insights on:
Human Karyotype Worksheet
Chromosome study: A karyotype is a visual presentation of the chromosomes present in each cell, usually derived from blood lymphocytes. The cells are grown in tissue culture, then arrested in the stage of separation (mitosis), then dropped on a glass slide from about 2 feet, which breaks them open; a dye is added to make stain them, a photo taken, then arranged into their 23 pairs, then studied for anomalies. ...Read more
Karyotyping is a test to examine chromosomes in a sample of cells, which can help identify genetic problems as the cause of a disorder or disease. This test can:
•count the number of chromosomes
•look for structural changes in chromosomes
the test can be performed on almost any tissue, including:
•placenta. ...Read more
One sample at a time: A blood sample is spun down and the white cells are separated into at least 3 tissue culture media sets. An amnio sample is spun down, the cells resuspended and divided into three tissue culture sets. Of primary importance: only one sample is ever set up at a time. No other sample is near another until the cultures have been completely set up and labeled. That prevents mixing up two babies by mistake ...Read more
What happens if we assume that the vitiligo is generic disorder then can we check if it's a dominant, recesive and carrier etc. using karyotyping.?
Autoimmune: Vitiligo is usually considered an autoimmune problem. If you want to find out whether it is genetic, you will need to get a detailed history on family members with this problem. I am not aware of any genetic markers uncovered thus far. Nonetheless if there has been no history of such a disorder among your immediate family members within 3 generations, genetic study would be meaningless. ...Read more
Not always: The decision to do an amniocentesis in order to do karyotyping is a personal decision and carries up to a 1 in 200 miscarriage rate. The information obtained would classically be used to decide upon termination or not as there is no treatable or correctable chromosome abnormality. At 35 years old and above, the risk of a chromosome defect is 1 in 200 or greater. ...Read more
What's my chance to have normal baby if me or my husband have balanced translocation&we already had a ds baby? We didn't do karyotyping yet though
Genetic counselor: You need to have a karyotype done before you can determine your risk. It would be very important for you and your spouse to see a genetic counselor to be able to better understand your risk. ...Read more
It stops clotting...: ...Without damaging or killing blood cells.Get a more detailed answer ›
Yes, but...: A karyotype is an expensive test - about $2000 dollars depending on what lab it is sent. Check with your insurance to see if they require pre authorization (ie approval for the diagnosis that your doctor is using the test for). If you have the test done without pre authorization on most plans, they would not cover it, and then the patient is responsible for the fees. ...Read more
Are there karyotyping like genetic level tests to confirm if a couple is compatible & will certainly have healthy children if they plan for pregnency?
Not really: There are no guarantees of compatibility or healthy children. The best thing to do is make sure that you and your partner are healthy and get early prenatal care if she becomes pregnant. If she intends to get pregnant, have her start taking a Folic Acid supplement (found in many vitamins) before she conceives. ...Read more
For what reason antibiotics are used in preparation of media for karyotyping using giemsa banding?
Basically tissue : culture, antibiotics used to prevent bacterial contamination during the cell growth prior to the preparation of the karyotypes. ...Read more
What's my chance to conceive with ds baby if me or my husband have the balanced translocation in karyotyping?
When karyotyping test to be done.I just had abortion. Is it to be done just before planning for pregnancy or immediately after abortion?
Talk with your OB-GY: That is a good question. I know your ob-gyn can better answer for you. ...Read more
My daughter was diagnosed with t cell all last year.Her karyotyping :abnormality in t(11;14)(p13;q11.2). Is there a targetted therapy for lmo2 issues?
Not yet: Gamma c gene inserted in scid patients; could cure the immune deficiency. But the two, gamma c gene was inserted close to lmo2 gene ( considered oncogene) which gets activated with gamma c gene insertion - causing increased proliferation on t cells ( causing t cell leukemia). Studies/ research on its way to target lmo2 gene. ...Read moreSee 1 more doctor answer
Wife pregnancy was terminated in 3rd month and karyotyping shows chromosomal analysis of poc is found abnormal 47, --+21. Is it will solve next time ?
2 early MCs, heartbeat seen in 2nd case, no live birth, turner's synd in fetus 2nd case, karyotyping & antiphosopolipid normal. Way fwd? More tests?
Try again: Abnormal chromosome patterns are seen in many of the MC's studied, so this would not be unexpected. If both you and spouse have been karyotyped & normal, you are not carrying a translocation that would doom you both to expect MC's.Consult the clinical genetics department at Toronto Children's if you have not already and see if their is anything more they recommend. ...Read moreSee 1 more doctor answer
3 early MCs no live birth. 32yrs. All tests normal autoimmune, antiphosopolipid, hormonal, karyotyping. Turner's in 1 fetus. Should we try again & how?
Yes: Don't give up, get followed by infertility/hi risk obstetrics doctor, at one point a medical geneticist input might be needed. For genetic / chromosomal disorders. PIGD, Pre- Implantation Genetic Diagnosis can be resorted to so as not to implant an embryo with a known major genetic/chromosomal disease, good luck ...Read more