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Doctor insights on: Hiv Elisa Positive Western Blot Negative

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My ELISA HIV test came up negative after 1month exposure. How accurate is this test?

My ELISA HIV test came up negative after 1month exposure. How accurate is this test?

90%: There's a period of time after a person is infected during which they won't test positive. This is called the “hiv window period.”

The window period can be from 9 days to 3-6 months, depending on the person's body and on the HIV-test that's used. ...Read more

Dr. Mathew Chengot
16 Doctors shared insights

Hiv (Definition)

Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more


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How reliable is a HIV ELISA test vs a western blot?

How reliable is a HIV ELISA test vs a western blot?

HIV testing: Elisa assay is quite sensitive (will detect antibodies best) but the western blot is more specific and confirm the results of the elisa if it was positive, as being either truly positive or false-positive. There are several other circumstances in which conditions other than HIV will produce a positive elisa assay. If there are symptoms and too early for antibody test, then PCR for RNA is best. ...Read more

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Is an HIV ELISA test always done with an western blot test?

Is an HIV ELISA test always done with an western blot test?

No: In general, the elisa is done first, and the lab then does a western blot if the elisa is positive. However, it is possible to order just the western blot, in which case the elisa usually isn't done. ...Read more

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Tested false positive ELISA HIV test last month. Negative ifa and western blot and naat. 2 negative oraquick all 1 1/2 year post exposure. Hiv?

Tested false positive ELISA HIV test last month. Negative ifa and western blot and naat. 2 negative oraquick all 1 1/2 year post exposure. Hiv?

Negative: If nat was negative at 6 months after exposure you not likely to have hiv. Given all the negative test results, you should not worry about the one apparently false positive. ...Read more

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2009- ELISA HIV test- positive, western blot- neg. 2010-oraquick negative. 2014- elisa-indeterminate wb- neg. Low risk for hiv. Could I have lupus?

2009- ELISA HIV test- positive, western blot- neg. 2010-oraquick negative. 2014- elisa-indeterminate wb- neg. Low risk for hiv. Could I have lupus?

Not a test for lupus: The HIV test is not a test for lupus. What's clear is that you don't have hiv. You'll never know why your elisa was positive and then indeterminate, and it's not worth investigating. False positive elisas are not uncommon; that's why they have to be confirmed with western blot. ...Read more

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False positive ELISA HIV test. Neg western blot, neg ifa &naat. I've had 2 neg oraquick home test. Could I be an elite controller? Or already have aids?

False positive ELISA HIV test. Neg western blot, neg ifa &naat. I've had 2 neg oraquick home test. Could I be an elite controller? Or already have aids?

No: Western blot is fairly definitive. You have 2 negative home tests and you are quite stressed about your testing:
her's an idea: use condoms: protect yourself (and others) at all times. ...Read more

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2 false pos ELISA HIV tests, all 1 1/2 years post exposure. Neg western blot, ifa, naat and 2 neg oraquick home tests. What is my HIV status? Help!

2 false pos ELISA HIV tests, all 1 1/2 years post exposure. Neg western blot, ifa, naat and 2 neg oraquick home tests. What is my HIV status? Help!

Likely negative: There is typically a "window" of 3-6 months after exposure where you could be HIV positive but test negative. Since you didn't get your testing until well after that, i'd say the confirmatory tests (western blot, ifa) are what you should go by. The elisa is very sensitive and can have false positives- that's why we rely on the confirmatory tests. I would consider your status to be negative. ...Read more

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Wht cause a false pos ELISA HIV test with neg naat and western blot? 2 neg oral fluid rapid results. After 1 year exposur. Ihave autoimmune hypotyroid

No answer: Please. How many times are you going to ask the same question. There is no right answer here. Your body has something triggering the simple HIV antibody test to be positive. It is not HIV causing this symptom. In the future you should avoid these tests and get the better test. You have been given this answer numerous times. It is time to move on. ...Read more

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False pos ELISA HIV tests, neg naat viral load test, neg western blot, ifa and oraquicks neg too. All 15 months post risk event. What should I do?

False pos ELISA HIV tests, neg naat viral load test, neg western blot, ifa and oraquicks neg too. All 15 months post risk event. What should I do?

Be grateful, man!: You've been obsessing about this too long. Sit down and talk with your physician about fears. If you'll forgive my frankness, talk with him/her and some wise adults (maybe even dad) about your emerging sexuality and how you've explored. By your age, we've all made mistakes. Embrace being a man, decide how you want love to be for you, and cherish adult love while resolving to be safe ; kind. ...Read more

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I just read that they do a confirmatory test called a western blot after the ELISA HIV test. Why?

I just read that they do a confirmatory test called a western blot after the ELISA HIV test. Why?

Confirm the results: Hiv elisa test is a screening test and has a few false positive resuilts.
Western blot test looks for and confirms that HIV genetic material is in the sample. ...Read more

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HIV, Elisa + january to july. WB New Lav Blot - in march. Cobas Taqman RNA - in march. Rapid tests for Ab - jan-jul, Geenius Confirmatory assay - july?

HIV: I am not sure if I understand your question correctly. But if you have a positive HIV Elisa and a Positive HIV WB, you have confirmed HIV infection. You will need to see an HIV specialist for treatment consideration.
I hope that helps. ...Read more

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When tested for hiv, how should a strongly positive ELISA test (index above 7) and a negative western blot be interpreted? What additional testing is required?

Likely negative: This is probably a negative test. If the results haven't changed in a month, then you're definitely not infected. If you don't want to wait that long, you could get a viral load test. An undetectable viral load would be highly reassuring, but i'd still repeat the serology. ...Read more

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Should I get an ELISA or western blot test if my hiv1&2 rapid tests come out negative? Hiv rapid is reliable?

PCR: The reason I would urge you to get the pricey pcr is that is is clear that you are worried, and it is costing you emotional energy. Pcr is the gold standard and will give you the right answer before either of the others. I hope things turn out well, but having HIV is no longer a death sentence. Good luck, live and love responsibly, and keep taking a proactive approach to your health. ...Read more

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With todays ultra sensitive modern ELISA tests, what use is the western blot for hiv? A few months ago I received a weakly reactive cmia HIV ag/ab test, a 4th generation HIV combo test, and this was followed by a negative western blot and a negative p24 t

With todays ultra sensitive modern ELISA tests, what use is the western blot for hiv? A few months ago I received a weakly reactive cmia HIV ag/ab test, a 4th generation HIV combo test, and this was followed by a negative western blot and a negative p24 t

False-positives: Screening tests are designed to be highly sensitive so they don't miss anyone with the disease. However, they can be so sensitive that they occasionally give a false-positive result. That is, the test appears positive for someone without the disease. The western blot, being time consuming and extra cost, is only used to make sure a positive is really a positive. Talk with your doc for more info. ...Read more

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Hi doctor. Is eli a more developed ELISA test for HIV? And is ecl a western blot test? (if yes for ecl) I had a negative p24 at 3 month am I negative

Hi doctor. Is eli a more developed ELISA test for HIV? And is ecl a western blot test? (if yes for ecl) I had a negative p24 at 3 month am I negative

Not Best Focus-Vague: Acronyms are too vague & not clear. However, the bigger issue is attributing too much significance to any specific test, all of which have issues of both false positives & negatives, never perfect. The larger issues are both probability, look up "bayes' theorem" & the many variables/complexity of exposure combined with individual responses over time. Before >6 months, reliability of HIV tests low. ...Read more

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My brother had a positive ELISA test for HIV followed by an indeterminate western blot. His viral load detected no virus. How confident should I be?

Pretty confident: It sounds like's he's negative and just happens to have an indeterminate wb. This happens sometimes, for reasons that are never entirely explained. However, I would recommend that he get the elisa and western blot retested one month after the first. If there has been no progression of the WB (addition of new bands), then he's definitely negative. ...Read more

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So there are no tests other than ELISA and western blot that would detect HIV if a person is sure of an encounter with an hiv+ person?

Repeat testing: It may take up to 6mos after an exposure to HIV for the ELISA & western blots to turn positive. If you were exposed to HIV & had negative tests, it is recommended that you get retested in 6 months. If positive, your doctor will perform more detailed studies, like viral load & CD4 counts. Not all exposures result in HIV infection, however you should use precautions to protect yourself & others. ...Read more

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2 + elisa, 1 -western blot, 1 -ifa and -natt, 2 -oraquick hometest all 1 year after exposure. Is HIV my problem?

HIV testing: No, but you no longer should ever do the HIV antibody test. If your doctors are not giving you the HIV pcr test then you may need to change who is testing you if you are worried about HIV exposure. ...Read more

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Would having MS cause a false positive HIV ELISA test?

Would having MS cause a false positive HIV ELISA test?

No: It will not. An elisa needs to be confirmed by a more specific test. ...Read more

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Does getting false positive HIV ELISA suggest there is something wrong with the patient? Or can the patient be healthy and get these results?

You're fine: I believe you have asked this before and been reassured. A false-positive elisa isn't a danger signal. Be thankful for good health, and go about your life. ...Read more

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I've had HIV Duo ultra tests after 14, 19, 31, 44, 54 days and anti-HIV (elisa) test at 46 and 60 days of exposure, all negative, Am I negative for hiv1-2?

Results conclusive: The HIV duo test is conclusive any time 4 weeks or more after the last possible exposure. All our tests after 31 days were superfluous. You're definitely negative for both HIV1 and HIV2. ...Read more

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Would HPV cause a false positive HIV ELISA test?

It shouldn't: Hpv shouldn't cause a false positive elisa. But see what the follow up western blot shows. ...Read more

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Are false positive HIV ELISA tests a sign of HSV 2?

Are false positive HIV ELISA tests a sign of HSV 2?

Please get help: Please call your therapist. You obviously do not believe anything we tell you here. Don't understand why you think it is okay to take our time up when you don't believe our answers are accurate. Please find another forum to deal with your internal struggles. ...Read more

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The odds of a false positive HIV ELISA test?

Unlikely: All tests are subject to prior probability (the likelihood of a condition
before any tests) and a refined probability, modified by test results.
That being said the HIV eia test is among the most accurate in medicine, and when confirmed by a western blot, approaches 100%

dr ted butler. ...Read more

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Is 12 weeks hiv Elisa negative test accurate?

See below: It is good that it is negative but it is too soon to exclude HIV. Get a HIV nucleic acid test and if that is negative at 6 months after exposure that would essentially exclude HIV.
For good health - Have a diet rich in fresh vegetables, fruits, whole grains, low fat milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex.
Get HPV vaccine. ...Read more

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How accurate is hiv Elisa testing after 12 weeks if negative?

Conclusive: At 12 or more weeks after the last possible exposure, negative ELISA is conclusive: 100% certain you do not have HIV. Congratulations! Stay safe. ...Read more

Dr. Ed Friedlander
1,495 Doctors shared insights

Human Immunodeficiency Virus (Definition)

Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids), [1][2] a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more


Elisa (Definition)

Enzyme-linked immunosorbent assay (elisa) is a test that uses antibodies and color change ...Read more