Doctor insights on:
Hiv Elisa Positive Western Blot Negative
There's a period of time after a person is infected during which they won't test positive. This is called the “hiv window period.”
The window period can be from 9 days to 3-6 months, depending on the person's body and on the HIV-test that's used. ...Read more
Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more
I am wondering about HIV results. I have tested at 5 months post exposure with dna/pcr, antibody, western blot test (negative). Is this conclusive?
HIV testing: Elisa assay is quite sensitive (will detect antibodies best) but the western blot is more specific and confirm the results of the elisa if it was positive, as being either truly positive or false-positive. There are several other circumstances in which conditions other than HIV will produce a positive elisa assay. If there are symptoms and too early for antibody test, then PCR for RNA is best. ...Read more
No: In general, the elisa is done first, and the lab then does a western blot if the elisa is positive. However, it is possible to order just the western blot, in which case the elisa usually isn't done. ...Read more
Tested false positive ELISA HIV test last month. Negative ifa and western blot and naat. 2 negative oraquick all 1 1/2 year post exposure. Hiv?
2009- ELISA HIV test- positive, western blot- neg. 2010-oraquick negative. 2014- elisa-indeterminate wb- neg. Low risk for hiv. Could I have lupus?
Not a test for lupus: The HIV test is not a test for lupus. What's clear is that you don't have hiv. You'll never know why your elisa was positive and then indeterminate, and it's not worth investigating. False positive elisas are not uncommon; that's why they have to be confirmed with western blot. ...Read more
False positive ELISA HIV test. Neg western blot, neg ifa &naat. I've had 2 neg oraquick home test. Could I be an elite controller? Or already have aids?
Western blot is fairly definitive. You have 2 negative home tests and you are quite stressed about your testing:
her's an idea: use condoms: protect yourself (and others) at all times. ...Read more
2 false pos ELISA HIV tests, all 1 1/2 years post exposure. Neg western blot, ifa, naat and 2 neg oraquick home tests. What is my HIV status? Help!
Likely negative: There is typically a "window" of 3-6 months after exposure where you could be HIV positive but test negative. Since you didn't get your testing until well after that, i'd say the confirmatory tests (western blot, ifa) are what you should go by. The elisa is very sensitive and can have false positives- that's why we rely on the confirmatory tests. I would consider your status to be negative. ...Read more
Wht cause a false pos ELISA HIV test with neg naat and western blot? 2 neg oral fluid rapid results. After 1 year exposur. Ihave autoimmune hypotyroid
No answer: Please. How many times are you going to ask the same question. There is no right answer here. Your body has something triggering the simple HIV antibody test to be positive. It is not HIV causing this symptom. In the future you should avoid these tests and get the better test. You have been given this answer numerous times. It is time to move on. ...Read more
False pos ELISA HIV tests, neg naat viral load test, neg western blot, ifa and oraquicks neg too. All 15 months post risk event. What should I do?
Be grateful, man!: You've been obsessing about this too long. Sit down and talk with your physician about fears. If you'll forgive my frankness, talk with him/her and some wise adults (maybe even dad) about your emerging sexuality and how you've explored. By your age, we've all made mistakes. Embrace being a man, decide how you want love to be for you, and cherish adult love while resolving to be safe ; kind. ...Read more
I just read that they do a confirmatory test called a western blot after the ELISA HIV test. Why?
When tested for hiv, how should a strongly positive ELISA test (index above 7) and a negative western blot be interpreted? What additional testing is required?
Likely negative: This is probably a negative test. If the results haven't changed in a month, then you're definitely not infected. If you don't want to wait that long, you could get a viral load test. An undetectable viral load would be highly reassuring, but i'd still repeat the serology. ...Read more
Should I get an ELISA or western blot test if my hiv1&2 rapid tests come out negative? Hiv rapid is reliable?
PCR: The reason I would urge you to get the pricey pcr is that is is clear that you are worried, and it is costing you emotional energy. Pcr is the gold standard and will give you the right answer before either of the others. I hope things turn out well, but having HIV is no longer a death sentence. Good luck, live and love responsibly, and keep taking a proactive approach to your health. ...Read more
With todays ultra sensitive modern ELISA tests, what use is the western blot for hiv? A few months ago I received a weakly reactive cmia HIV ag/ab test, a 4th generation HIV combo test, and this was followed by a negative western blot and a negative p24 t
False-positives: Screening tests are designed to be highly sensitive so they don't miss anyone with the disease. However, they can be so sensitive that they occasionally give a false-positive result. That is, the test appears positive for someone without the disease. The western blot, being time consuming and extra cost, is only used to make sure a positive is really a positive. Talk with your doc for more info. ...Read more
Does a negative hiv-1 western blot at 6 months (no ELISA was completed only wb) indicate no hiv-2 as well? Or should I screen again?
Western blot: Most sensitive test available. Negative excellent.Get a more detailed answer ›
Hi doctor. Is eli a more developed ELISA test for HIV? And is ecl a western blot test? (if yes for ecl) I had a negative p24 at 3 month am I negative
Not Best Focus-Vague: Acronyms are too vague & not clear. However, the bigger issue is attributing too much significance to any specific test, all of which have issues of both false positives & negatives, never perfect. The larger issues are both probability, look up "bayes' theorem" & the many variables/complexity of exposure combined with individual responses over time. Before >6 months, reliability of HIV tests low. ...Read more
My brother had a positive ELISA test for HIV followed by an indeterminate western blot. His viral load detected no virus. How confident should I be?
Pretty confident: It sounds like's he's negative and just happens to have an indeterminate wb. This happens sometimes, for reasons that are never entirely explained. However, I would recommend that he get the elisa and western blot retested one month after the first. If there has been no progression of the WB (addition of new bands), then he's definitely negative. ...Read more
So there are no tests other than ELISA and western blot that would detect HIV if a person is sure of an encounter with an hiv+ person?
Repeat testing: It may take up to 6mos after an exposure to HIV for the ELISA & western blots to turn positive. If you were exposed to HIV & had negative tests, it is recommended that you get retested in 6 months. If positive, your doctor will perform more detailed studies, like viral load & CD4 counts. Not all exposures result in HIV infection, however you should use precautions to protect yourself & others. ...Read more
Why would an e-hiv or ELISA test come back "repeatedly reactive" when the secondary western blot test comes back non-reactive? What can cause this?
False positive?: You might want to get a viral load to be sure. ...Read more
Very reliable: Elisa is to screen, western blot is to confirm. ...Read more
No: It will not. An elisa needs to be confirmed by a more specific test. ...Read more
Does getting false positive HIV ELISA suggest there is something wrong with the patient? Or can the patient be healthy and get these results?
You're fine: I believe you have asked this before and been reassured. A false-positive elisa isn't a danger signal. Be thankful for good health, and go about your life. ...Read more
I've had HIV Duo ultra tests after 14, 19, 31, 44, 54 days and anti-HIV (elisa) test at 46 and 60 days of exposure, all negative, Am I negative for hiv1-2?
All tests are subject to prior probability (the likelihood of a condition
before any tests) and a refined probability, modified by test results.
That being said the HIV eia test is among the most accurate in medicine, and when confirmed by a western blot, approaches 100%
dr ted butler. ...Read more
It is good that it is negative but it is too soon to exclude HIV. Get a HIV nucleic acid test and if that is negative at 6 months after exposure that would essentially exclude HIV.
For good health - Have a diet rich in fresh vegetables, fruits, whole grains, low fat milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex.
Get HPV vaccine. ...Read more
Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids),  a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more
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