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Doctor insights on: Hiv 1 Rna Quant R T Pcr Csf

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Dr. Adam Lewis
16 doctors shared insights

Hiv (Definition)

Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more


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Is my 8 wks 2 day PCR RNA + 4th Gen test negative conclusive for HIV infection after vaginal exp? Or do i need to test after 12 wks or it has to be after 13 wks? Pls answer, Thanks for any response

Is my 8 wks 2 day PCR RNA + 4th Gen test negative conclusive for HIV infection after vaginal exp? Or do i need to test after 12 wks or it has to be after 13 wks? Pls answer, Thanks for any response

Relax, Mike: I understand that you're a man worried about HIV after an unprotected episode of intercourse with a woman. The nucleic acid tests are almost always positive by this time -- we can't even estimate the frequency of their turning positive only at 12 weeks because history isn't always accurate. Retest only if you or someone close to you is obsessed over it. Love wisely and well from now on. Cheers. ...Read more

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What is the window period for p42 antigen/ antibody dual 4th gen HIV test? And the window period for HIV RNA PCR test? When can it be conclusive?

What is the window period for p42 antigen/ antibody dual 4th gen HIV test? And the window period for HIV RNA PCR test? When can it be conclusive?

See below: Please consult this site for a detailed answer. Nucleic acid tests are conclusive about a month after acquiring infection. http://www.sfaf.org/hiv-info/testing/hiv-test-window-periods.html ...Read more

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Given that HIV 1 RNA PCR test result undetectable 25 days after possible exposure, when and which HIV test to take next?

HIV: If you are negative by HIV RNA PCR at 25 days after exposure the chances of you becoming HIV positive are next to nil. If you really want to test yourself a combination test which includes p24 antigen and HIV antibody can be done at 3 months. Think you are going to be fine. ...Read more

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What is the level of sensitivity of the HIV-1 RNA PCR test by Aptima? Is it accurate at 2 to 3 weeks post exposure?

What is the level of sensitivity of the HIV-1 RNA PCR test by Aptima? Is it accurate at 2 to 3 weeks post exposure?

Yes: Read the CDC updated recommendations on HIV testing. The rationale for this test being available is that HIV RNA is detectable in plasma before antibodies. A safe approach is to be tested after the eclipse period after potential exposure when no RNA nor antibodies to HIV can be detected. The RNA test is effective around 10 days after infection but seroconversion to antibodies may take 3 months. ...Read more

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Can hiv-1 RNA PCR detect hiv-2 at some point or can not detect?If you consider Poland, can you exclude HIV possibility with hiv-1 RNA PCR test only?

Can hiv-1 RNA PCR detect hiv-2 at some point or can not detect?If you consider Poland, can you exclude HIV possibility with hiv-1 RNA PCR test only?

HIV Online: We are very experienced here with what we call HIV panic. There is little we will say that will ease your panic. HIV 1 in the disease you are at risk for in Poland in general. Your HIV RNA test is negative. If you cannot get past this worry then go get an HIV 1/2 antibody test with the P24 antigen test as well. Then test every twelve weeks. And if you are at risk get on Truvada (emtricitabine and tenofovir) to prevent. ...Read more

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Hiv-1 RNA PCR result is undetected,worried about hiv-2 in Germany. Dr didnt see necessary to take antibody test even at w12.What should I do?

Hiv-1 RNA PCR result is undetected,worried about hiv-2 in Germany. Dr didnt see necessary to take antibody test even at w12.What should I do?

Wait: You must wait. Or get an HIV 1/2 antibody test but know you will have to repeat it in the future. At twelve weeks it cannot be 100% accurate. in the future if you have a high risk exposure there is a treatment to prevent infection if started within 72 hours. If you have repeated high risk exposures there is a treatment to take before exposure to prevent infection. ...Read more

Polymerase Chain Reaction (Definition)

Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other ...Read more


Dr. Ed Friedlander
1,417 doctors shared insights

Human Immunodeficiency Virus (Definition)

Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids), [1][2] a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more