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Doctor insights on: Hiv 1 Rna Quant R T Pcr Csf

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Polymerase Chain Reaction (Definition)

Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other ...Read more


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HIV NEGATIVE combo (antibody + antigen) tests at 4,6,10,13,14 weeks and PCR RNA VL UNDETECTED (14 weeks) white tongue, chronic pharyngitis. Am I HIV-?

HIV NEGATIVE combo (antibody + antigen) tests at 4,6,10,13,14 weeks and PCR RNA VL UNDETECTED (14 weeks) white tongue, chronic pharyngitis. Am I HIV-?

You are HIV negative: Listen, man. Stop doing this to yourself. Get to an otolaryngologist and see if you have a post-nasal drip, maybe heavy snoring without realizing it, or some actual serious illness. Try scraping the stuff off your tongue with a piece of dental floss; if it's still white patches, ask your dentist's opinion also. But please get over these HIV fears. Maybe even be thankful for having shared love. ...Read more

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HIV NEGATIVE combo (antibody + antigen) tests at 4,6,10,13,14 weeks and PCR RNA VL UNDETECTED (14 weeks) white tongue. Risk: gay deep kiss. Am I HIV-?

HIV NEGATIVE combo (antibody + antigen) tests at 4,6,10,13,14 weeks and PCR RNA VL UNDETECTED (14 weeks) white tongue. Risk: gay deep kiss. Am I HIV-?

You are HIV-neg: I hope this is good news. "White tongue" is usually bacteria, and you may be able to scrape them off with a piece of dental floss held tight. You can trust the test if it is your only exposure. I hope you're comfortable with your sexuality and that you're pleased to have shared a kiss -- guys of all orientations may enjoy perhaps kissing a buddy, and nobody's ever gotten HIV by kissing. ...Read more

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Hep B Virus (HBV) DNA Qualitative (Detection) - COBAS TaqMan , Hep C RNA Detection by Taqman detection will be conclusive on 47 days? Any retest need?

Hep B Virus (HBV) DNA Qualitative (Detection) - COBAS TaqMan , Hep C RNA Detection by Taqman detection will be conclusive on 47 days? Any retest need?

No: Both Hepatitis B and C viruses may be detected 30 to 60 days after infection by antigen detection or 60-120 days with antibody testing. Direct HBV DNA and HCV RNA testing can potentially detect infection earlier, within 2-4 weeks after infection, so being tested at 47 days post-exposure with a negative nucleic acid test for both is very likely to be a true negative ...Read more

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Advia hep.C antibody +, HCV RNA -. 3 months later Architect hep C Ab -, HCV RNA -. 2 Hep C Ab Verification tests -.Is Advia hepC Ab test false positiv

Advia hep.C antibody +, HCV RNA -. 3 months later Architect hep C Ab -, HCV RNA -. 2 Hep C Ab Verification tests -.Is Advia hepC Ab test false positiv

Without more info: and only the data you submitted in your question, I would put it together the way you did: the first Advia hepC Ab test was a false positive. I presume other experts who arranged your follow-up testing have suggested the same interpretation. Stay well:) ...Read more

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(-)4th gen hiv 1.5,2.3.4.5.5.5 mnth, DNA PCR 10,12 wk, RNA PCR 18 wk, vdrl,hsv,hcv,chlmydia 11,16 wk. R these 100% conclusive after risky exposure?

(-)4th gen hiv 1.5,2.3.4.5.5.5 mnth, DNA PCR 10,12 wk, RNA PCR 18 wk, vdrl,hsv,hcv,chlmydia 11,16 wk. R these 100% conclusive after risky exposure?

Stop testing!: You have been seriously overtested, especially for HIV. The 4th gen HIV tests are conclusive any time 4 weeks or more after exposure. All HIV tests after the one at 1.5 months were superfluous and a waste of money and time. The other tests also are conclusive and also didn't need to be done a second time. No matter how high the risk might have been, you can move on without worry. ...Read more

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Hep c anti body 14 with cutoff of 1, but qualitative HCV RNA PCR says no RNA material detected. In notes says single test not enough . My alt, cbc, ok

Hep c anti body 14 with cutoff of 1, but qualitative HCV RNA PCR says no RNA material detected. In notes says single test not enough . My alt, cbc, ok

Probably cleared: The hcv viral load tests are not fda approved to confirm active infection, although this is how most doctors use it. If a repeat viral load in around 4 months is also negative, people are considered to have spontaneously cleared infection. ...Read more

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My hepb serology hbasg +ve, confirmatory test hbsag-ve, hbcab, hbsab, hbeag, hbeab are all -ve, this is 3rd tym same result, am I infected?

My hepb serology hbasg +ve, confirmatory test hbsag-ve, hbcab, hbsab, hbeag, hbeab are all -ve, this is 3rd tym same result, am I infected?

My vote is...: No, based on the confirmatory results that antigens and antibodies are negative. As the surface antibody (HBsAb) is currently negative, one can ask his primary care for Hepatitis B vaccines, to acquire protection. However, if one was exposed recently, and got a new infection, he could be in the brief window period, if the HBcAb test was for IgG only (thus missing HBcAb IgM). His Dr. can explain. ...Read more

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Hiv Test- 85 & 121 days 4th Gen test and RT RNA PCR negtiv. 149 days HIV 1/O/2 Ab ( 3rd gen) negtiv. is this conclusiv or do i need more testing? ongoing symps - lymph nodes for 3 mons. pls ans, thks

Hiv Test- 85 & 121 days 4th Gen test and RT RNA PCR negtiv. 149 days HIV 1/O/2 Ab ( 3rd gen) negtiv. is this conclusiv or do i need more testing? ongoing symps - lymph nodes for 3 mons. pls ans, thks

Not for HIV: It would help to know your risk factors for acquiring HIV, but, regardless, absence if viral DNA after 4 months followed by absence of HIV antibody at 5 months means that we're 99.9% certain you haven't acquired the HIV virus. However, you need to follow up with your doctor about the swollen lymph nodes. ...Read more

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False pos ELISA HIV tests, neg naat viral load test, neg western blot, ifa and oraquicks neg too. All 15 months post risk event. What should I do?

False pos ELISA HIV tests, neg naat viral load test, neg western blot, ifa and oraquicks neg too. All 15 months post risk event. What should I do?

Be grateful, man!: You've been obsessing about this too long. Sit down and talk with your physician about fears. If you'll forgive my frankness, talk with him/her and some wise adults (maybe even dad) about your emerging sexuality and how you've explored. By your age, we've all made mistakes. Embrace being a man, decide how you want love to be for you, and cherish adult love while resolving to be safe ; kind. ...Read more

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Oral sex with unknown, continuous 5month low grade fever, lymph nodes, sore throat, 107 da HIV eia negative HIV PCR RNA not detected, what other std?

Oral sex with unknown, continuous 5month low grade fever, lymph nodes, sore throat, 107 da HIV eia negative HIV PCR RNA not detected, what other std?

NOT STD: As you have been told this is not an STD. But, it could be an infectious disease. That list is hundreds of possibilities long and will include testing for lymphoma, leukemia, other blood cell cancers, and all of the possible non-STD infectious diseases. It is time to sign off of the internet and to see your doctor for the appropriate testing or infectious disease consultation. ...Read more

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Hello these are my lab results got yesterday after tested positive for HBsAG 1 week back.Hbe ag neg, anti hbe ag positive & HBV DNA quant 570. ?

Hello these are my lab results got yesterday after tested positive for HBsAG 1 week back.Hbe ag neg, anti hbe ag positive & HBV DNA quant 570. ?

Hepatitis B: All laboratory test results need to be interpreted in the clinical context and the doctor who ordered the tests is usually in the best position to do that. Having said that, you have active hepatitis B infection and should see your doctor for follow-up care. ...Read more

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Hep B surface Ab - positive, Hep BE antigen - positive, Viral DNA 2000 IU. Acute Hep B DX. Tests don't match the serology timeline? Am I in recovery?

Need expert care: This situation is too complex for safe advice on this forum. You should be seeing an expert in person, or perhaps seek HealthTap's concierge service with an infectious diseases or liver disease expert. ...Read more

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Advia hep. C antibody +,HCV RNA -. 3 months later Architect hep. C antibody -, HCV RNA -. 2 hep C Ab Verification tests-.Can above person donate blood?

Advia hep. C antibody +,HCV RNA -. 3 months later Architect hep. C antibody -, HCV RNA -. 2 hep C Ab Verification tests-.Can above person donate blood?

May be: You ought to disclose these results to the blood bank so that they may make a determination on your eligibility to donate along with other health factors. I commend you for considering doing your civic duty. ...Read more

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CMIA 4th gen antibody screening test (not antigen) for hiv 1 & 2 @50 days is 0.15 non reactive.Is antibody screening without antigen accurate @7thweek?

CMIA 4th gen antibody screening test (not antigen) for hiv 1 & 2 @50 days is 0.15 non reactive.Is antibody screening without antigen accurate @7thweek?

Conclusive at 6+ wk: Official advice by many agencies is that stand-alone HIV antibody tests are not conclusive until 3 months. However, with the modern tests in current use, including the test you had, the results actually are conclusive any time 6 or more weeks after exposure. Feel free to have another test at 3 months if another negative result will help you get beyond your fears. But i really isn't necessary. ...Read more

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Is my 8 wks 2 day PCR RNA + 4th Gen test negative conclusive for HIV infection after vaginal exp? Or do i need to test after 12 wks or it has to be after 13 wks? Pls answer, Thanks for any response

Is my 8 wks 2 day PCR RNA + 4th Gen test negative conclusive for HIV infection after vaginal exp? Or do i need to test after 12 wks or it has to be after 13 wks? Pls answer, Thanks for any response

Relax, Mike: I understand that you're a man worried about HIV after an unprotected episode of intercourse with a woman. The nucleic acid tests are almost always positive by this time -- we can't even estimate the frequency of their turning positive only at 12 weeks because history isn't always accurate. Retest only if you or someone close to you is obsessed over it. Love wisely and well from now on. Cheers. ...Read more

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What is the window period for p42 antigen/ antibody dual 4th gen HIV test? And the window period for HIV RNA PCR test? When can it be conclusive?

What is the window period for p42 antigen/ antibody dual 4th gen HIV test? And the window period for HIV RNA PCR test? When can it be conclusive?

See below: Please consult this site for a detailed answer. Nucleic acid tests are conclusive about a month after acquiring infection. http://www.sfaf.org/hiv-info/testing/hiv-test-window-periods.html ...Read more

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Is hiv 1 rna pcr qualitative and elisa 3rd gen test reliable after 4.5 months of exposure. both came not detected?

Is hiv 1 rna pcr qualitative and elisa 3rd gen test reliable after 4.5 months of exposure. both came not detected?

100% reliable: Either of these tests, by itself, proves 100% you don't have HIV. There is no chance you are infected. Stop testing and stop worrying about HIV, no matter what exposure or symptoms you may have. ...Read more

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Given that HIV 1 RNA PCR test result undetectable 25 days after possible exposure, when and which HIV test to take next?

HIV: If you are negative by HIV RNA PCR at 25 days after exposure the chances of you becoming HIV positive are next to nil. If you really want to test yourself a combination test which includes p24 antigen and HIV antibody can be done at 3 months. Think you are going to be fine. ...Read more

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What is the level of sensitivity of the HIV-1 RNA PCR test by Aptima? Is it accurate at 2 to 3 weeks post exposure?

Yes: Read the CDC updated recommendations on HIV testing. The rationale for this test being available is that HIV RNA is detectable in plasma before antibodies. A safe approach is to be tested after the eclipse period after potential exposure when no RNA nor antibodies to HIV can be detected. The RNA test is effective around 10 days after infection but seroconversion to antibodies may take 3 months. ...Read more

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Can hiv-1 RNA PCR detect hiv-2 at some point or can not detect?If you consider Poland, can you exclude HIV possibility with hiv-1 RNA PCR test only?

Can hiv-1 RNA PCR detect hiv-2 at some point or can not detect?If you consider Poland, can you exclude HIV possibility with hiv-1 RNA PCR test only?

HIV Online: We are very experienced here with what we call HIV panic. There is little we will say that will ease your panic. HIV 1 in the disease you are at risk for in Poland in general. Your HIV RNA test is negative. If you cannot get past this worry then go get an HIV 1/2 antibody test with the P24 antigen test as well. Then test every twelve weeks. And if you are at risk get on Truvada (emtricitabine and tenofovir) to prevent. ...Read more

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Hiv-1 RNA PCR result is undetected,worried about hiv-2 in Germany. Dr didnt see necessary to take antibody test even at w12.What should I do?

Hiv-1 RNA PCR result is undetected,worried about hiv-2 in Germany. Dr didnt see necessary to take antibody test even at w12.What should I do?

Wait: You must wait. Or get an HIV 1/2 antibody test but know you will have to repeat it in the future. At twelve weeks it cannot be 100% accurate. in the future if you have a high risk exposure there is a treatment to prevent infection if started within 72 hours. If you have repeated high risk exposures there is a treatment to take before exposure to prevent infection. ...Read more

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I had 7 hiv duo test at 3rd 4th 6th 8th 12.7th 13.3 th 15th 1) antiobdy 9.5 week 1) hiv 1 rna pcr qualitative at 14.5 week All -Non reactive hiv?

Stop testing!: You have been seriously overtested for HIV. The duo test is always positive within 4 weeks of infection. Every test after that was superfluous and a waste of money. It is past time to move on without further worry about it. ...Read more

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Does cobas taqman v 2 for HIV 1 RNA check for all groups N, O, P, M and all subtypes of M. If not, what it doesn't check. Please answer, Thanks?

A technical question: I am looking at the Roche website regarding this test and it says this test, "Quantifies the clinically significant HIV-1 groups and subtypes with full subtype coverage and quantifications of HIV-1 groups O and M" It is a confirmatory, not screening test. I believe you would need to contact the company if you need more specific information than this. Good wishes:) ...Read more

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First unprotected sex on 9th June. Antibody test on 14th June & 14 July. Aptima HIV-1 RNA, Qualitative, TMA on 20th may. All negative.Should I worry?

Repeat in 6 months: Just to be sure repeat the HIV nucleic acid test 6 months after the June episode. I trust you got tested for other STDs as well. Wish you good health. ...Read more

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Is a HIV ag/ab eia and a hiv-1 Rna test conclusive at 5 months ? I also had a 4th gen test 3 months ago both negative. Blood was cold if that matters

HIV negative: If your fourth generation test and RNA tests are both negative at 5 months after an HIV exposure, as long as you haven't had any other exposures in the meantime, then yes you would be considered HIV-negative. ...Read more

Dr. Milroy Samuel
16 doctors shared insights

Hiv (Definition)

Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more


Dr. Ed Friedlander
1,498 doctors shared insights

Human Immunodeficiency Virus (Definition)

Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids), [1][2] a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more