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Doctor insights on: Hiv 1 Hiv 2 Western Blot Immunoblot

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Dr. Creighton Wright
16 doctors shared insights

Hiv (Definition)

HIV is also known as HIV/AIDS. Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more


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What is the definition or description of: HIV 1/hiv 2 western blot?

What is the definition or description of: HIV 1/hiv 2 western blot?

Immunoblot: Western blot is an antibody detection test. Viral antigens (the protein coat of hiv) are immobilized in a matrix (gel). These are then transferred to Cellulose membrane and a persons serum is exposed to it. Antibodies in the serum then bind to the proteins (ie gp120). Antibodies that do not attach are washed away. Chemicals are then applied to allow visualization of the bands. ...Read more

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If i had HIV 2 would my HIV 1 western blot be intermediate after 1 year of exposure? I got a negative western blot and naat test for hiv.

MIstreatment?: Any doctor who diagnosed you with a positive HIV test should be sending the HIV RNA test and not just the western blot test. You are in the U.S. You need a smarter or more experienced doctor for this. You have been left out to worry for over one year and it appears not once been given the right test. I don't know your personal life story and what your exposure was. ...Read more

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When is it necessary to have a hiv-1 western blot assay?

EIA +: The test is automatically done by the lab if the eia (elisa) antibody test is positive. People with positive rapid tests should also have their results confirmed by standard eia/wb serology. ...Read more

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Could one infected with both hiv-1 and hiv-2 at a time?

Yes: Infection with either hiv-1 or hiv-2 is not protective against a superimposed infection by the other virus. So in theory, yes, one could get both in one individual. Hiv-2 is fairly uncommon in the us so infection with it is unlikely. ...Read more

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Can the inst hiv-1/hiv-2 antibody rapid test be reliable after 62 days to possible HIV exposure? Test results were negative. Could this be reliable?

HIV Testing: No, unfortunately. For the routine HIV antibody tests you need a minimum of three months or 90 days and a safe view of six months. However there is testing that is much more reliable. This is the HIV pcr dna testing. This test, if negative after 21 days post exposure is 100% accurate if negative. ...Read more

Dr. Ed Friedlander
1,372 doctors shared insights

Human Immunodeficiency Virus (Definition)

Human immunodeficiency virus is also known as HIV/AIDS. Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids), [1][2] a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more