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Doctor insights on: Her2 Elisa

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What is an elisa? I had an ELISA test, but I don't understand what it is.

What is an elisa? I had an ELISA test, but I don't understand what it is.

Radiation-free test: Elisa is a test that detects a foreign substance (antigen) or an antibody in blood. Elisa uses an enzyme bound to a 2nd antibody . In the final step the enzyme converts indicator material to a different color. The amount of color detected corresponds to the amount of antigen or antibody present. Elisa gives the same answer as rast without using radioactive radioisotopes. ...Read more

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Elisa (Definition)

Enzyme-linked immunosorbent assay (elisa) is a test that uses antibodies and color change ...Read more


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Is there any facility to confirm HSV 2 in india like glycoprotin test i got ELISA negtiv 2times and once IgG positiv please tell me laboratry name plz?

Is there any facility to confirm HSV 2 in india like glycoprotin test i got ELISA negtiv 2times and once IgG positiv please tell me laboratry name plz?

HSV 2: You cannot differentiate between hsv types 1 and 2 using antibody testing because of the high frequency of cross-reactivity. The only sure way to do this is to isolate the virus from active lesions and test the virus for serotypes. This is fairly sophisticated, but imagine most large medical schools in india may have a lab that can do this. ...Read more

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I have to take a ELISA test today for Lyme disease but i was just wondering how does ELISA test work?

I have to take a ELISA test today for Lyme disease but i was just wondering how does ELISA test work?

Sandwich assay...yum: Elisa = enzyme-linked immuno sorbent assay. Done by adding serum from a subject to a plate w/antibody against the infection you're testing for on its surface. The antigen (or protein) from the infection binds to the antibody on the plate. Then another antibody to the infection w/color signal chemical attached is added on top and binds to the antigen. If infection is there color can be measured. ...Read more

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Is it better to be her 2 positive or her2 negative?

Is it better to be her 2 positive or her2 negative?

Her2 positive: Breast cancer is broken into 4 types - each involves the presence or absence of estrogen, progesterone, and her 2 receptors: luminal a (er/pr positive, her 2 negative), luminal b (er/pr positive, her 2 positive), her 2 (er/pr negative, her 2 positive), triple-negative (er/pr negative, her 2 negative). Patients who are her 2 positive herceptin, (trastuzumab) a powerful drug that greatly improves survival. ...Read more

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How do you measure anti-dna antibody? Using elisa?

How do you measure anti-dna antibody? Using elisa?

Anti-DNA antibodies: Simple blood tests can be performed to test for anti-dna antibodies. Usually the screen is started with a basic ANA test which can then be followed with more specific antibody testing to determine if a specific disease can be identified. Of course, symptoms must also correlate since we do not simply provide diagnosis based on lab findings. ...Read more

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My ELISA anti rubella IgG negative(0.13 ul/ml) and ELISA anti rubella IgM positive(>400). Is it safe to pregnancy. ?

My ELISA anti rubella IgG negative(0.13 ul/ml) and ELISA anti rubella IgM positive(>400). Is it safe to pregnancy. ?

No: Igm rubella indicates recent exposure and body response to it. It does not refer immunity. Hence baby at risk for congenital rubella. ...Read more

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What are the benefits we get from zometa and what are the side effect? I'm a her2 breast cancer stage 3 patient

What are the benefits we get from zometa and what are the side effect? I'm a  her2 breast cancer stage 3 patient

Controversial: I personally think there is a benefit based on the trials. Specifically in post-menopausal women. Not only treating or preventing osteoporosis but also preventing breast cancer recurrence. But i think the final answer is still unknown. The side effects are few but one which is rare can be problematic, osteonecrosis of the jaw. ...Read more

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Prolonged fever(>7w)+swollen painful lymph nodes(neck and behind the ears)+headache. Tested -ve for HIV(ELISA 1&2),Hep-B&C,VDRL what else could it be?

Prolonged fever(>7w)+swollen painful lymph nodes(neck and behind the ears)+headache. Tested -ve for HIV(ELISA 1&2),Hep-B&C,VDRL what else could it be?

Many causes: There are many causes for swollen lymph nodes and fever. Remember there is a window period for HIV testing - about 3 months and hepatitis up to 12 weeks! Other causes could be infectious mononucleosis, fungal infections, TB, even cancer. You need close follow up with your doctor for more testing. Good luck ...Read more

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Hiv Rapid test and Elisa past 12 weeks of exposure.Results negative.D o i need to test again?Please reply me.

Hiv Rapid test and Elisa past 12 weeks of exposure.Results negative.D o i need to test again?Please reply me.

HIV Testing: According to the CDC website you should be tested one more time at 6 months from exposure. ...Read more