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Doctor insights on: Her2 Elisa

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What is an elisa? I had an ELISA test, but I don't understand what it is.

What is an elisa? I had an ELISA test, but I don't understand what it is.

Radiation-free test: Elisa is a test that detects a foreign substance (antigen) or an antibody in blood. Elisa uses an enzyme bound to a 2nd antibody. In the final step the enzyme converts indicator material to a different color. The amount of color detected corresponds to the amount of antigen or antibody present. Elisa gives the same answer as rast without using radioactive radioisotopes. ...Read more

Elisa (Definition)

Enzyme-linked immunosorbent assay (elisa) is a test that uses antibodies and color change ...Read more


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Is there any facility to confirm HSV 2 in india like glycoprotin test I got ELISA negtiv 2times and once IgG positiv please tell me laboratry name plz?

Is there any facility to confirm HSV 2 in india like glycoprotin test I got ELISA negtiv 2times and once IgG positiv please tell me laboratry name plz?

HSV 2: You cannot differentiate between hsv types 1 and 2 using antibody testing because of the high frequency of cross-reactivity. The only sure way to do this is to isolate the virus from active lesions and test the virus for serotypes. This is fairly sophisticated, but imagine most large medical schools in india may have a lab that can do this. ...Read more

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I have to take a ELISA test today for Lyme disease but I was just wondering how does ELISA test work?

I have to take a ELISA test today for Lyme disease but I was just wondering how does ELISA test work?

Sandwich assay...yum: Elisa = enzyme-linked immuno sorbent assay. Done by adding serum from a subject to a plate w/antibody against the infection you're testing for on its surface. The antigen (or protein) from the infection binds to the antibody on the plate. Then another antibody to the infection w/color signal chemical attached is added on top and binds to the antigen. If infection is there color can be measured. ...Read more

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Is it better to be her 2 positive or her2 negative?

Is it better to be her 2 positive or her2 negative?

Her2 positive: Breast cancer is broken into 4 types - each involves the presence or absence of estrogen, progesterone, and her 2 receptors: luminal a (er/pr positive, her 2 negative), luminal b (er/pr positive, her 2 positive), her 2 (er/pr negative, her 2 positive), triple-negative (er/pr negative, her 2 negative). Patients who are her 2 positive herceptin, (trastuzumab) a powerful drug that greatly improves survival. ...Read more

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How do you measure anti-dna antibody? Using elisa?

How do you measure anti-dna antibody? Using elisa?

Anti-DNA antibodies: Simple blood tests can be performed to test for anti-dna antibodies. Usually the screen is started with a basic ANA test which can then be followed with more specific antibody testing to determine if a specific disease can be identified. Of course, symptoms must also correlate since we do not simply provide diagnosis based on lab findings. ...Read more

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My ELISA anti rubella IgG negative (0.13 ul/ml) and ELISA anti rubella IgM positive (>400). Is it safe to pregnancy.?

My ELISA anti rubella IgG negative (0.13 ul/ml) and ELISA anti rubella IgM positive (>400). Is it safe to pregnancy.?

No: Igm rubella indicates recent exposure and body response to it. It does not refer immunity. Hence baby at risk for congenital rubella. ...Read more

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What are the benefits we get from zometa and what are the side effect? I'm a her2 breast cancer stage 3 patient

What are the benefits we get from zometa and what are the side effect? I'm a her2 breast cancer stage 3 patient

Controversial: I personally think there is a benefit based on the trials. Specifically in post-menopausal women. Not only treating or preventing osteoporosis but also preventing breast cancer recurrence. But I think the final answer is still unknown. The side effects are few but one which is rare can be problematic, osteonecrosis of the jaw. ...Read more

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I went to a doctor, cz I feeling pain on left side of body, lik nerve issue. He tested my ESR and told no need of ELISA test. Both of them have relation?

No: Elisa is an incomplete answer, and is related to a type of testing, not the actual test itself. Esr is just the rate of sedimentation of your red cells, and is a general measure of inflammation. ...Read more

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Prolonged fever (>7w)+swollen painful lymph nodes (neck and behind the ears)+headache. Tested -ve for HIV (ELISA 1&2), Hep-B&C, VDRL what else could it be?

Prolonged fever (>7w)+swollen painful lymph nodes (neck and behind the ears)+headache. Tested -ve for HIV (ELISA 1&2), Hep-B&C, VDRL what else could it be?

Many causes: There are many causes for swollen lymph nodes and fever. Remember there is a window period for HIV testing - about 3 months and hepatitis up to 12 weeks! Other causes could be infectious mononucleosis, fungal infections, TB, even cancer. You need close follow up with your doctor for more testing. Good luck ...Read more

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Hiv Rapid test and Elisa past 12 weeks of exposure. Results negative. D o I need to test again? Please reply me.

Hiv Rapid test and Elisa past 12 weeks of exposure. Results negative. D o I need to test again? Please reply me.

HIV Testing: According to the CDC website you should be tested one more time at 6 months from exposure. ...Read more

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What is the difference between an ANA and ELISA test? I tested high positive for one and negative for the other. Please explain in simplest terms. TY!

What is the difference between an ANA and ELISA test? I tested high positive for one and negative for the other. Please explain in simplest terms. TY!

ANA vs ELISA: Well ANA is a protein produced by our immune system in various situation. ELIZA is method which uses to identify and detect ANA. There is also another method used to detect ANA. In 2nd method the titer is reported too rather then saying it's positive or negative. ...Read more

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On Mitochondrial antibody test by Elisa, 0-20 neg, 21-24.9equivocal, 25&>pos? Would a level of 29 be a low positive?

On Mitochondrial antibody test by Elisa, 0-20 neg, 21-24.9equivocal, 25&>pos? Would a level of 29 be a low positive?

Yes: The decision to treat for primary biliary cirrhosis, or just to watch, will be based on several factors, one of which is the level of the AMA. ...Read more

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My ELISA HIV test came up negative after 1month exposure. How accurate is this test?

My ELISA HIV test came up negative after 1month exposure. How accurate is this test?

90%: There's a period of time after a person is infected during which they won't test positive. This is called the “hiv window period.”

The window period can be from 9 days to 3-6 months, depending on the person's body and on the HIV-test that's used. ...Read more

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What is the definition or description of: elisa?

Lab test: Enzyme-linked immunosorbent assay (elisa) is a test that uses antibodies and color change to identify a substance. ...Read more

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What are the differences between ELISA and immunoassay?

What are the differences between ELISA and immunoassay?

ELISA one type of: Immunoassay. Enzyme Linked Immuno-Sorbent Assay is one type of immunoassay. Others are agglutination, flocculation, precipitation, turidimetry, nephelometry, radio-immunoassay, etc. Pregnancy test is one example of an immunoassay. ...Read more

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Ya me hice la prueba de elisa de vih y salio negativa pero desde mi relación de riesgo no se me quita el dolor de garganta y granos en la lengua?

Probable no VIH: The HIV blood tests are among the most accurate diagnostic tests ever developed, for any medical condition. If you were tested long enough after your last exposure (6 weeks for most standard tests), the results overrule both exposure hsitory and symptoms. If you were tested beyond 6 weeks, for sure you don't have HIV. Also, spots on the tongue and sore throat are not typical HIV symptoms. ...Read more

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For what HBSAg spot ELISA test taken?

Hepatitis B: Presence of HbSAg indicates active hepatitis B viral replication in the body and an active infection. It can and should be treated. ...Read more

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Hiv 7months ELISA test negative 100% accurate?

No: If positive, it will be followed up with a confirmatory test. If negative, you are almost certainly okay but if you are very worried you might be able to pay for a pcr assay, which is the gold standard. ...Read more

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How can someone with HIV consistently test negative using elisa?

How can someone with HIV consistently test negative using elisa?

How do you know HIV: If HIV antibody testing is negative through ELISA how do you know the person is hiv+? If they have been shown to have HIV by PCR testing for viral RNA it is possible, but unlikely, that their immune system is so impaired that they cannot form antibodies. It is also possible, but again unlikely, that they have HIV type ii and not being tested for this. Something does not make sense. ...Read more

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What is the definition or description of: influenza on serology test (elisa)?

Unreliable test: Refers to a test that determines the presence of antibodies against the influenza virus. It has a very inconsistent sensitivity rate, and can potentially read a negative result in a patient that actually does have the flu. Superceded by molecular techniques that are more accurate but expensive to administer. ...Read more

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Apart from elisa, which other immunoassay (s) can be used for antigen analysis?

Apart from elisa, which other immunoassay (s) can be used for antigen analysis?

Precipitation, . ..: Earliest tests were based on antigen-antibody binding resulting in precipitation. Other indicators of antibody-antigen binding are agglutination, complement binding, enzyme interference etc. ...Read more

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Apart from elisa, which other immunoassay (s) can quantitative antigen analysis use?

See below: Rocket electrophoresis, radial immunodiffusion, conventional radio-immunoassy, densitometric scanning. There are many variations of ELISA itself. ...Read more

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Has MS caused false positive ELISA for people? Is it common for MS to interfere with elisa?

Has MS caused false positive ELISA for people? Is it common for MS to interfere with elisa?

Not at all: Do not know which condition was being tested, but MS does not affect outcomes of common testing for infection, metabolic disorders, or co-morbidity searches. ...Read more

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What are probabilties to have HIV after testing negative in a 4th gen ELISA 30 days after exposure?

What are probabilties to have HIV after testing negative in a 4th gen ELISA 30 days after exposure?

Need additional test: Most people will develop a positive test within 8 weeks following exposure. But... At 30 days the number is significantly less.

Need to repeat test at 3 and 6 months to be absolutely certain.

In the meanwhile be absolutely safe in your sex. ...Read more

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I have to take a ELISA test today for Lyme disease but I was just wondering how does ELISA test work?

Sandwich assay...yum: Elisa = enzyme-linked immuno sorbent assay. Done by adding serum from a subject to a plate w/antibody against the infection you're testing for on its surface. The antigen (or protein) from the infection binds to the antibody on the plate. Then another antibody to the infection w/color signal chemical attached is added on top and binds to the antigen. If infection is there color can be measured. ...Read more

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What to do if I have to take a ELISA test today for Lyme disease but I was just wondering. 1. how does ELISA test work?

What to do if I have to take a ELISA test today for Lyme disease but I was just wondering. 1. how does ELISA test work?

Just show up: You just show up and have your blood drawn. If your Lyme screening test is positive, they will automatically do more specific antibody testing on your blood to determine if you have active Lyme disease. ...Read more

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Thanks alot. What is the difference between HIV Negative Elisa tests 0.18 and 0.36? was 0.18 2 months ago and now 0.36.please help

Thanks alot. What is the difference between HIV Negative Elisa tests 0.18 and 0.36? was 0.18 2 months ago and now 0.36.please help

Meaningless: These are lab values that express to the lab authorities the validity of the test and do not in any way represent anything of concern to you. These both represent being HIV negative. They are both fully negative and the 'numbers' are meaningless for you to worry about. ...Read more

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Elisa test after 3 weeks&5 weeks negative. Only deep French kissing no penteration at all. Is it OK or not enough. I'm really worry?

No risk at all: Presumably you're speaking of HIV risk and testing. You were not at risk and did not need testing; HIV is not transmitted by kissing, deep or otherwise. (HIV is not transmitted by saliva.) And if your ELISA was for the antigen-antibody (4th generation) test, the 5 week result is conclusive. If a stand-alone antibody test, the results are over 95% conclusive, maybe have a final test at 6 wk. ...Read more