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Doctor insights on: Gram Stain Chart

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Dr. Linda Callaghan
25 doctors shared insights

Gram Stain (Definition)

The two major families of common bacteria are distinguished by the chemistry of their walls, specifically the ability of iodine to mordant their staining with crystal violet and prevent its being removed with strong solvent. Gram positives include staph and strep, while gram negatives include e. Coli. The stain has been in use for around 150 years and remains the ...Read more


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Pls interpret vagina gram stain reslt.Gm Stain (-),Polymorphonuclear-Moderate,Epi cells-Moderate,Gm(+)Cocci pairs-Few,Gm(+)bacili-Mod,Gm(-)bacili-rare?

Pls interpret vagina gram stain reslt.Gm Stain (-),Polymorphonuclear-Moderate,Epi cells-Moderate,Gm(+)Cocci pairs-Few,Gm(+)bacili-Mod,Gm(-)bacili-rare?

Probably normal: It's hard to give you a definitive answer without seeing it and knowing what was causing your diarrhea, but the vagina normally has bacteria present (all the Gm + and - categories) and some polymorphonuclear cells (neutrophils). The gram+ bacilli are most likely lactobacillus, the normal flora of the vagina.The epithelial cells are simply the lining of the vagina. ...Read more

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Pls interpret result in my gram stain.Gm Stain (-),Polymorphonuclear-Moderate,Epi cells-Moderate,Gm(+)Cocci pairs-Few,Gm(+)bacili-Mod,Gm(-)bacili-rare

Pls interpret result in my gram stain.Gm Stain (-),Polymorphonuclear-Moderate,Epi cells-Moderate,Gm(+)Cocci pairs-Few,Gm(+)bacili-Mod,Gm(-)bacili-rare

Gram stain: You don't mention what the specimen is but I'm guessing it's a sputum sample? Looks like mixed flora, probably normal oropharyngeal flora, pretty nonspecific. ...Read more

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Positive for fungal spores gram stain:few gram negative bacilli in singles, few gram cocci in singles and moderate epithelial cells & pus cells noted ?

Sputum?: This sounds like a grossly inadequate sputum specimen, or one which was actually primarily saliva. The organisms seen are all part of normal oral flora, and this specimen should be repeated with an induced sputum specimen if the clinical situation warrants. ...Read more

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SPUTUM Gram Stain1+ Squamous Epithelial Cells 2+ Polymorphonuclear Leukocytes 2+ Mixed Gram Positive Organisms 2+ Normal Flora Is this normal. ?

 SPUTUM 
Gram Stain1+ Squamous Epithelial Cells
2+ Polymorphonuclear Leukocytes
2+ Mixed Gram Positive Organisms 
2+ Normal Flora
Is this normal.
?

Non diagnostic : The squamous epithelium means the sputum came from mouth not lungs (no squamous in lungs/bronchi) but leukocytes means infection. So, this sputum has infection but can't use it to diagnose due to contamination from mouth normal saliva that has usual mouth bacteria (called normal flora). Actinomycosis is being appropriately treated with penicillin. Discuss with your doc to see if need sputum resent ...Read more

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What is this test for :CULT & GRAM STAIN SPUTUM.

What is this test for :CULT & GRAM STAIN SPUTUM.

Infection?: Gram stain of any body fluid or tissue is for microscopic visualization of bacteria and other organisms. Cult refers to "culture" which is where the tissue or fluid sample is placed in a special medium that allows any organisms that may have infected the tissue to grow. This allows clear identification of which if any organisms are causing infection. ...Read more

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CULT & GRAM ST AEROBE & ANAEROBE What this result mean?1+ Gram Positive Cocci Chains No WBCs.

Culture/Gram's stain: Depends upon whether these were from the culture or the stain of some clinical material, where the culture was obtained from, and why. This may be an entirely inconsequential result or possibly represent severe illness, but would need far more info to say. Discuss this with the doctor who cultured you. If this was from your tonsil it is probably Strep and you should have been treated. Get better. ...Read more

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Spotted fever gram stain?

Gram Negative: Gram-negative bacteria are bacteria that do not retain crystal violet dye in the gram staining protocol. In a gram stain test, a counterstain is added after the crystal violet, coloring all gram-negative bacteria with a red or pink color. - wikipedia r. Rickettsii are gram negative bacteria. ...Read more

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What does a Vaginal gram stain of many epithelial cells , moderate WBCs seen, many gram positive rods, & moderate gram positive cocci indicate?

Bacterial vaginosis: is an overgrowth of hodge podge of bacteria in the vaginal vault. This overgrowth can lead to unpleasant odor, discharge and abdominal pain . BV can be treated easily most of the time. ...Read more

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Sputum culture showed normal flora.gram stain showed mod.amt of gram positive and neg. bacilli and mod.amt of gram positive cocci.few wbc.concerns?

Sputum culture showed normal flora.gram stain showed mod.amt of gram positive and neg. bacilli and mod.amt of gram positive cocci.few wbc.concerns?

No.: From your description, it sounds like the diagnosis was normal flora present. The bacilli and cocci with few WBCs are just describing that. Normal flora is a mixed population like that described. When one has an infection, the causative organism will predominate and will likely be the only thing present. There will also usually be numerous WBCs as these are your cells that fight infection. ...Read more

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Doctors, what is the difference between gram positive cocci & gram negative bacilli?

Doctors, what is the difference between gram positive cocci & gram negative bacilli?

Gram stain: The gram stain has been used for a long time to classify bacteria according to the chemistry of their cell walls (and hence whether they are gram positive or negative.) Cell shape and growth characteristic are also useful in grouping bacteria until more full speciation information becomes available. The gram stain is fast, reasonably reproducible by trained personnel and the groupings useful 4 rx ...Read more

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CULT & GRAM STAIN SPUTUM ,is this type of test is good to look for actinomyces ,mold and fungis?I

CULT & GRAM STAIN SPUTUM ,is this type of test is good to look for  actinomyces ,mold and fungis?I

Need more info: This organism can be difficult to isolate since they are facultative anaerobes, but the Gram's stain of clinical materials is usually very very suggestive, depending upon the site from which the specimen was obtained. This is a common cause of osteomyelitis of the bones of the jaw. Routine cultures are not a good way to look for molds and other fungi except for yeast-like fungi. Good luck. ...Read more

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What's a rare gram negative rods mean? It was taken vaginally via gram stain.

This could be normal: Bacteria are classified, broadly, as being gram-negative or gram-positive (retains violet dye color after staining--gram stain-- a lab slide with specimen on it). And either being rod (cylindrical/barrel shaped) or spherical (cocci) when viewed under a microscope. Gram negative rods are very common in the genital tract, and it sounds like you don't have too many. I say probably normal. Good wishes ...Read more

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How can a gram-positive coccus appear after gram staining?

How can a gram-positive coccus appear after gram staining?

As a sphere: A solitary sphere or as in the case of streptococci as a chain or as in staphlococci as a bunch like grapes on a vine. This is streptococcus under the scanning elctron microscope. ...Read more

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Wondering what my sinus culture means? Gram Stain: Many polys Mod squamous epith cells Rare pos cocci  

Wondering what my sinus culture means?
Gram Stain: Many polys
Mod squamous epith cells
Rare pos cocci
 

Sinus culture: Sinus culture shows growth of microorganisms such as bacterial or fungus etc. If culture is positive for some microorganism, then it can be treated accordingly. Squamous cells can be seen in sinus contents. ...Read more

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Gram stain sputum- presence of few epithelial cells, moderate number of pus cells,few gram positive cocci in chain observed in smear What does this in?

Sputum gram stain: Sputum gram stain testing is not done nearly as often as it once was, since we now know it usually isn't very helpful in diagnosis. The white blood cells (pus cells) suggest infection like bronchitis or pneumonia -- but it's usually possible to judge this just by appearance of the sputum (e.g., yellow or green mucus). Gram pos cocci could be entirely normal bacteria. Discuss with your doctor. ...Read more

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What is meant by gram positive bacteria and gram negative bacteria ?

What is meant by gram positive bacteria and gram negative bacteria ?

Staining technique: Gram positive organisms have a high amount of material in their cell walls which retain gentian violet (a purple stain) on slides looked at under the microscope. Gram negative organisms have the gentian violet washed out when subjected to acid-alcohol and then they are restained with a red stain which renders them red under the microscope. It is one way of loosely classifying bacteria. ...Read more

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Gram Stain Result Few Polys Rare Gram negative rods N>10,000 CFU/mL normal upper respiratory flora present no squamous epithelial cells seen?

Context: gram negative rods not so common and that seems a large amt. This implies an infection requiring particular antibiotics and this is not the more common cause of sinus/bronchitis but Hemophilus ...Read more