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Doctor insights on: False Negative Rna Pcr Hiv

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What is the likelihood of a false negative on a PCR RNA TMA HIV test at 15-20 days post PEP/post exposure? I have heard of false positives.

What is the likelihood of a false negative on a PCR RNA TMA HIV test at 15-20 days post PEP/post exposure? I have heard of false positives.

Too soon to rule: Out HIV. The PCR RNA test if negative at about 3 months after exposure would rule out infection. A negative result before than may not be definitive. A positive result be definitive any time. ...Read more

Polymerase Chain Reaction (Definition)

Polymerase chain reaction is a method of finding a specific piece of dna and copying it over and over again until there is enough to study. The technique allows a scientist to detect very small amounts of dna in a swab sample then reproduce enough of it to study it. It is very sensisitive for detecting the presence of certain bacteria and is also used in doing other ...Read more


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What are the % chances of having all false neg HIV 4th Gen test@7, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 wks, DNA PCR@10, 12 wks & RNA PCR@20 wks?

What are the % chances of having all false neg HIV 4th Gen test@7, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28 wks, DNA PCR@10, 12 wks & RNA PCR@20 wks?

Virtually nil: With this many tests giving negative results, you have far greater chance of getting killed by lightening than HIV.
For good health - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex. ...Read more

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Am I HIV free irrespective of a-z persisting symptoms, exposure; I have HIV PCR RNA 5, 15 week not det, ab 2, 8, 15, 28, 32 neg; or do I need more tests??

STD, EBV testing: You have had more than enough testing for for HIV. Typically, a sexually active person should have an HIV screen every 3-6 months.

Your symptoms suggest another types of infection: You should also be tested for EBV (Epstein Barr virus) and CMV (cytomegalovirus); both can cause mono-like symptoms. Testing for gonorrhea/chlamydia as well as syphilis are also important. ...Read more

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Is 59 days HIV combo + PCR RNA test neg conclusive? Some docs say yes, some say no and need to be tested at 3, 6, 12 months. What is correct? Some org say 12, 13 lots of confusing info Please answer!

Is 59 days HIV combo + PCR RNA test neg conclusive? Some docs say yes, some say no and need to be tested at 3, 6, 12 months. What is correct? Some org say 12, 13 lots of confusing info Please answer!

The 3, 6, 12 is old: Based on data that was purely antibody; even the CDC website screws that up.

"HIV testing may be concluded 4 months after exposure "

http://nccc. Ucsf. Edu/wp-content/uploads/2014/03/Updated_USPHS_Guidelines_Mgmt_Occupational_Exposures_HIV_Recommendations_PEP. Pdf

Thing is, nobody including me, believes you have ONLY ONE EXPOSURE lifetime. So, the real situation is to be tested about every 6-12 mo ...Read more

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Hiv rna I&2 qualitative pcr test after 10 days post receiving rimming negative. Is it reassuring?

Low risk, too soon: To test. Rimming, even if performed by a person with HIV is a relatively low risk exposure. To be sure, get a HIV nucleic acid test about 6 months after exposure.
Wish you good health! - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex, if you have sex. ...Read more

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I had a HIV RNA PCR and 4th generation test at 26 days post exposure. Results were negative, I have symptoms, how accurate are these results? Worried

I had a HIV RNA PCR and 4th generation test at 26 days post exposure. Results were negative, I have symptoms, how accurate are these results? Worried

Accurate but...: While the tests you have taken are good, the are not fool-proof. The standard is to retest at 6 months. This gives your body time to form antibodies. Be careful. ...Read more

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Just undergone HIV PCR 2 mos. After risk exposure. Result is NO DETECTABLE LEVEL OF HIV RNA. Does this mean am negative in HIV? Can I stop worrying now?

Just undergone HIV PCR 2 mos. After risk exposure. Result is NO DETECTABLE LEVEL OF HIV RNA. Does this mean am negative in HIV? Can I stop worrying now?

Repeat in 4 months: You are in all likelihood free of HIV, just to make sure repeat the test in 4 months.
For good health - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex. ...Read more

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If 1hiv PCR RNA Quant test negative at 40, 105 days, is that enough to understand person has not got hiv?, or need more test on 6 mon? And What tests?

Probably enough: The definitive test to show no HIV infection is a negative antibody test and that should be done a minimum of 6 weeks after intercourse but usually more like 3-6 months. However if a pcr test is negative at 1 month and 3 months that's is essentially conclusive that you are negative, but you should have the Elisa and western blot antibody tests to be sure. ...Read more

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I had a HIV PCR RNA test done 14 days after high risk exposure and symptoms the result came back negative is this a good sign?

I had a HIV PCR RNA test done 14 days after high risk exposure and symptoms the result came back negative is this a good sign?

Yes: Repeat it in a few more weeks as your physician advises. Love safely from now on. And cherish having shared love, even if it was not perfect and did put you at risk. ...Read more

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How reliable is negative HIV PCR RNA test around 12 weeks after unprotected exposure? Do I need more testing? Thanks in advance

How reliable is negative HIV PCR RNA test around 12 weeks after unprotected exposure? Do I need more testing? Thanks in advance

Good to go, Mike: Be thankful for good health. Whatever happened, I hope you'll cherish a memory of having given and received love. Like all human love, it was imperfect and dangerous. Perhaps you're smarter from the experience -- it happens to all of us. Be wise, and perhaps there'll be someone soon with whom you'll find lifetime true love. ...Read more

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4 month PCR RNA HIV negative and 4, 5, 6 month 4th Gen Abbott Architect AG/AB negative, should I retest? Am I HIV free? How PCR works? Is it reliable?

4 month PCR RNA HIV negative and 4, 5, 6 month 4th Gen Abbott Architect AG/AB negative, should I retest? Am I HIV free? How PCR works? Is it reliable?

Since you are neg.: For both the HIV virus itself (PCR RNA) and the antibody to HIV (AG/AB), you can be confident that you don't have HIV. PCR tests for HIV RNA by multiplying the number of RNA fragments to detectable levels. AG/AB testing detects your immune systems response to HIV. Taken together, all these NEG tests are very reliable. Time to focus on behavior that may have put you at risk. Dr. Anne. ...Read more

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Is my 8 wks 2 day PCR RNA + 4th Gen test neg conclusive for HIV infec after vagi exp? Or do I need to test after 12 r 13 wks? Thanks for any response

No more HIV testing: The 4th gen blood test (for HIV antibody and p24 antigen) is considered conclusive any time 4 weeks or more after the last possible exposure. The RNA test was superfluous but also confirms you didn't catch HIV. Also, it is extremely rare to catch HIV from a single episode of vaginal sex in the US or western Europe, where HIV is rare even in the most sexually active women. All is well ...Read more

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Had unprtected vaginal sex (insertive).Had some symptoms after10 days Tested after 10 and 18 days rna pcr, 26 days hiv duo negative. Is it conclusive?

Too early to know: You could have taken post-exposure prophylaxis, however that window has passed. If the HIV nucleic acid test is negative at 12 weeks after exposure, that is more or less conclusive, provided you do not keep exposing yourself, ...Read more

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Would RNA PCR for HIV 1 n cmia duo detect subtype e n other rare subtypes also at 8 months post xposr n thailnd. Many smptms? Negative result coclusive?

Would RNA PCR for HIV 1 n cmia duo detect subtype e n other rare subtypes also at 8 months post xposr n thailnd. Many smptms? Negative result coclusive?

Yes, but...: The latest pcr tests for HIV should be able to detect the rarer subtypes, but in life, no test is perfect. Life is about chances (probabilities, likelihoods), so if it has been 8 months since a known high risk exposure to HIV and one's primary doctor (or infectious diseases doctor) has done adequate testing, then negative results mean one did not catch HIV that time. Keep in touch with the doctor. ...Read more

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Ultra sensitive RNA PCR HIV 48-10, 000, 000 not dected along with 2nd gen antibody neg also 32days gonna retest with home access in5 days should I worry?

Ultra sensitive RNA PCR HIV 48-10, 000, 000 not dected along with 2nd gen antibody neg also 32days gonna retest with home access in5 days should I worry?

Probably not: Would retest in 6 months, but the absence of measurable HIV RNA makes this highly improbable that you are infected. The better question is how and why were you exposed, and do you plan precautions in the future. You may not believe this, but people lie about their sexual activities and whether or not they have std's. Be a boy scout and be prepared! ...Read more

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Hi doctors, 20+ hiv 4th gen test (upto 14 months), 1 rna pcr (@20 weeks), 2 dna pcr (@11 & 18 week). All neg. Am I 100% free of Hiv or need more tests?

Hi doctors, 20+ hiv 4th gen test (upto 14 months), 1 rna pcr (@20 weeks), 2 dna pcr (@11 & 18 week). All neg. Am I 100% free of Hiv or need more tests?

Free: You can quit testing.
For good health - Have a diet rich in fresh vegetables, fruits, whole grains, milk and milk products, nuts, beans, legumes, lentils and small amounts of lean meats. Avoid saturated fats. Drink enough water daily, so that your urine is mostly colorless. Exercise at least 150 minutes/week and increase the intensity of exercise gradually. Do not use tobacco, alcohol, weed or street drugs in any form.
Practice safe sex, if you have sex. ...Read more

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Chances of 12 weeks negative 4th Gen combo and PCR RNA turning positive for HIV after an unprotected encounter with a female of unknown status? Thanks

Chances of 12 weeks negative 4th Gen combo and PCR RNA turning positive for HIV after an unprotected encounter with a female of unknown status? Thanks

Less than 1%: Multiple reports cite that the sensitivity (ability to accurately measure a truly negative test) of the 4th generation IV test is 100%. Examples given include 1 false negative/508 specimens, another 2 false negatives/669 tests. You are negative for HIV. ...Read more

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Had protected vaginal sex with CSW. How reliable is negative HIV p24 antigen combo test at 20 days mark? Also, getting results tomorrow for HIV RNA PCR at 19 days. Will that be conclusive if negative?

Had protected vaginal sex with CSW. How reliable is negative HIV p24 antigen combo test at 20 days mark? Also, getting results tomorrow for HIV RNA PCR at 19 days. Will that be conclusive if negative?

Pretty reliable: Most people who turn positive would be positive at least borderline by day 14-20. There are uncommon cases of over 1 month to turn positive. NOte that the incidence of this is far lower than the liklihood of someone Totally Lying about What They are Doing! .
Oh, sorry, that's for the antibody. The antigen/other measures of virus should be done TOGETHER with antibody. REALLY low risk of false negat ...Read more

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Is hiv rna quantitative pcr (hiv 1&2) after 10 days negative result conclusive? Is 10 days with in the window period of this test?

Is hiv rna quantitative pcr (hiv 1&2) after 10 days negative result conclusive? Is 10 days with in the window period of this test?

About 95% conclusive: This is almost but not quite conclusive. It is not the recommended test after a particular exposure, unless there are symptoms to suggest a new HIV infection. For a conclusive result, also have an HIV antibody tests (or combo test, i.e. antigen-antibody) 4 weeks after the event. The combination of that negative result plus PCR at 10 days will be conclusive. ...Read more

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If nurse made mistake (exposed to air or heat) taking blood, will that effect a HIV PCR RNA/VIRAL LOAD test? Negative result but worried. Pls help.

No: Occasionally on the broadcast news, somebody will find human remains that have been in the jungle or in the desert for months or years... and the crime lab will identify who died, by using DNA analysis. Testing for RNA or DNA will reliably detect the RNA or DNA that is being looked for, if it is there. ...Read more

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What is the accuracy of HIV testing (antibody and antigen) 6 weeks post PEP? I had all tests (Neg) including the pcr rna test at 22 days post pep.

What is the accuracy of HIV testing (antibody and antigen) 6 weeks post PEP? I had all tests (Neg) including the pcr rna test at 22 days post pep.

HIV testing & RNA: Read the CDC updated recommendations on HIV testing. The rationale for this test being available is that HIV RNA is detectable in plasma before antibodies. A safe approach is to be tested after the eclipse period after potential exposure when no RNA nor antibodies to HIV can be detected. The RNA test is effective around 10 days after infection but seroconversion to antibodies may take 3 months. ...Read more

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6 week HIV RNA negative. Anti-hcv negative <0.1 8 week HIV negative < HCV PCR negative. 10 week HIV negative HCV 0.2 negative.? Remain negative?

6 week HIV RNA negative. Anti-hcv negative <0.1 8 week HIV negative < HCV PCR negative. 10 week HIV negative HCV 0.2 negative.? Remain negative?

Likely yes: I think your in the clear for hep c. While its great that your negative HIV RNA pcr the typical protocol is time 0, 3 and 6 months after that. Now having said that...I have a large primarily HIV practice in south florida and its rare in my opinion not to have seroconverted and developed high titres of HIV virus at this point. But you still need further follow up. ...Read more

Dr. Lois Freisleben-Cook
16 Doctors shared insights

Hiv (Definition)

Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more


Dr. Ed Friedlander
1,496 Doctors shared insights

Human Immunodeficiency Virus (Definition)

Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids), [1][2] a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more