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Doctor insights on: Enzyme Assay Techniques

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How to measure anti-dna antibody using farr assay?

How to measure anti-dna antibody using farr assay?

Radioactivity: The farr assay is a radioimmunologic assay. It uses radiolabeled antigens to detect specific antibodies in serum. The antigens are allowed to react with the serum and then precipitated using a special reagent such as protein a sepharose beads. The bound radiolabeled immunoprecipitiate is commonly analzyed by gel electrophoresis. ...Read more

Enzyme (Definition)

An enzyme causes the transformation of a compoond to another compound. In living structures, it is the transformation of a protein to another form. An example is from an inactive state ...Read more


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Please interpret- C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

Please interpret-
C.trachomatis RNA TMA Result: Detected. Standard range: Not detected. Test performed using the Aptima Combo2 (R) assay gen-probe(r)

You have chlamydia: "Detected" means the test found chlamydia -- i.e. a positive result. Return to the doctor or clinic where the test was done for treatment. (If you tested yourself at a lab, see a doctor or clinic ASAP.) Your current or recent sex partner(s) also need treatment. Good luck! ...Read more

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Substrate name for lipase enzyme?

Substrate name for lipase enzyme?

Olive oil: At least that's what we used for assays when i was doing a lot of clinical chemistry. Today kitmakers sell you "lipase substrate" which the physician need only do quality control on. I suspect it's olive oil. ...Read more

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Apart from elisa, which other immunoassay(s) can quantitative antigen analysis use?

Apart from elisa, which other immunoassay(s) can quantitative antigen analysis use?

See below: Rocket electrophoresis, radial immunodiffusion, conventional radio-immunoassy, densitometric scanning. There are many variations of ELISA itself. ...Read more

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Enzyme inducers & enzyme inhibitors, what are these?

Enzyme inducers & enzyme inhibitors, what are these?

Biochemistry enzymes: One induces and other inhibits action of enzyme. Great Wikipedia question. If you have never heard of it always go there first before coming here. ...Read more

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HSV 1 positive through blood test =[LIAISON assay using chemiluminescence immunoassay (CLIA) tech.] Index=1.47, how likely is it false positive?

HSV 1 positive through blood test =[LIAISON assay using chemiluminescence immunoassay (CLIA) tech.]  Index=1.47, how likely is it false positive?

Not false positive: False positive tests for HSV1 are very rare. Half of all persons your age have positive tests, so nothing unusual there. Most likely from an oral infection in the distant past. ...Read more

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What are these tests done for? Hb, tc, ast, alt, bilurubin total, direct. Creatinine, quantitative CRP assay and rbs.

What are these tests done for?
Hb, tc, ast, alt, bilurubin total, direct. Creatinine, quantitative CRP assay and rbs.

General health: Just as a grown man, i might walk into my physician's office after several years of neglecting my annual physicals, and expect to get all of these checked. Health maintenance, fishing expedition, or focused workup -- all are common tests. ...Read more

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Will copegus (ribavirin) treatment increase liver enzyme levels?

Will copegus (ribavirin) treatment increase liver enzyme levels?

Copegus/liver enzyme: Some people may get worsening of liver functions including elevation of enzymes, bilirubin, brown urine and yellow eyes and medication has to be stopped immediately. ...Read more

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LDH Isoenzymes lab test results: LDH total- 153. LD1- 32, LD2-30, LD3, 20, LD4- 8, LD5- 10. Are these results normal? Is the LD1&LD2 ratio abnormal?

LDH Isoenzymes lab test results: 
LDH total- 153. LD1- 32, LD2-30, LD3, 20, LD4- 8, LD5- 10. Are these results normal? Is the LD1&LD2 ratio abnormal?

Historic test: Matthew, the LDH isoenzyme test is ancient history, almost never ordered nowadays. And your LDH is normal. The old 1-2 ratio is an antique screen for a heart attack, and it's neither sensitive nor specific. I know you're obsessing over the idea that you have lymphoma, and a few websites suggest this as a screening test -- but it's not. Focus on building a life for yourself. ...Read more

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Is there kind of test exists ? Enzyme Immunoassay (rapid) ??

Is there kind of test exists ?

Enzyme Immunoassay (rapid) ??

Yes: This.is an important class of labs used for a variety of purposes. Why do you ask? ...Read more

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Best serum tests for liver damage (fibrosis/cirr.)? Ggt, apo a1, a2 alpha gammaglobulin, ldh, haptoglobin? Others? How to interpret? Caff breath test?

Best serum tests for liver damage (fibrosis/cirr.)? Ggt, apo a1, a2 alpha gammaglobulin, ldh, haptoglobin? Others? How to interpret? Caff breath test?

Need more tests: Serum tests may never become abnormal from fibrotic or cirrhosis until late. Ultrasound abdomen is good. Ct may help. Liver biopsy is best. Usually clinical history helps. Nonalcoholic fatty liver is emerging as a new and potentially big problem and is mostly diagnosed incidentally on ct or ultrasound of abdomen. ...Read more

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TSH with human anti-mouse antigen lab test correlation and purpose ?

TSH  with human anti-mouse antigen lab test correlation and purpose ?

Unclear questions: Mouse antibodies are used in many immunoassays, including test for TSH. Could you please restate your questions as it is not clear in its present form. You may visit this site for information on TSH. http://www.nlm.nih.gov/medlineplus/ency/article/003684.htm ...Read more

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Is there kind of test exists ?Enzyme Immunoassay (rapid) ??

Lots: There are large number of enzyme immunoassays for many different chemicals, infectious agents,hormones, proteins. What test did you have in mind. ...Read more

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-incom. Rt. Bundle branch block -cholesterol (total), serum by enzymatic method 208 -biochemistry lipid profile : LDL cholesterol, serum 133 ?

-incom. Rt. Bundle branch block 
-cholesterol (total), serum by enzymatic method 208
-biochemistry lipid profile : LDL cholesterol, serum 133

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Unlikely 2B related: Total and LDL cholesterol look ok, unless you have heart disease or multiple risk factors for heart disease, then LDL goal is <100. Inocmplete rbbb can be normal. Sometimes is just actually a "r s r-prime" pattern in certain electrocardiogram leads- many times a normal finding seen in healthy young military recruits. Incomplete rbbb can progress to a complete right bundle block. ...Read more

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Spec.chemistry TEST NAME PSA SCREEN 0.18 then space is blank=1= FREE T4 1.18. TSH 1.863 COMM. =1=the PSA assay is a direct chemiluminometric immunoasy?

See below: All laboratory tests need to be interpreted in the clinical context and the doctor who ordered the tests is usually in the best position to do that. Having said that, the results you provided are all normal. ...Read more

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Globulin level: 4.5, Albumin level: 3.9, Total Protein: 8.4, Albumin/Globulin Ratio: 0.9, AST: 68, ALT: 105. What do these levels mean?

Globulin level: 4.5, Albumin level: 3.9, Total Protein: 8.4, Albumin/Globulin Ratio: 0.9, AST: 68, ALT: 105. What do these levels mean?

Need med history: You need to tell us why was this test done. Did you have some complaints? Once we know your symtoms, if any, it is easier to interpret your lab results. Your liver enzymes are slightly high and so is the globulin level which can occur due to liver problems like Hepatitis. Your doctor needs to recheck these labs in another one or two months and find the cause?hepatitis? ...Read more

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Are enzyme tablets effective for improving digestion? (e.g. amylase 120mg, protease 70mg, lipase 58mg, bromelain 100mg, etc) When should they be used?

Are you deficient?: Some folks have pancreatic lesions and cannot digest their food without supplemental enzymes. This is a common problem in kids with cystic fibrosis. For most folks, it's a complete waste of money -- like wearing glasses if you don't need them. ...Read more

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With bipolar ii, adhd, & c ptsd, what additional tests are beneficial? Ie: hormone, neurotransmitter , DNA mutation (mthfr, a1at ...) methylation etc.

Yes: I would definitely check mthfr and comt. Both these are involved in methylation. One is involved in neurotransmitter synthesis, and the other in breakdown. Therefore polymorphisms can impact mood, etc, and if so, vitamins and supplements can help the methylation cycle. I check mthfr in most of my patients. ...Read more

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