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Doctor insights on: Elisa Test For Hiv

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My ELISA HIV test came up negative after 1month exposure. How accurate is this test?

My ELISA HIV test came up negative after 1month exposure. How accurate is this test?

90%: There's a period of time after a person is infected during which they won't test positive. This is called the “hiv window period.” The window period can be from 9 days to 3-6 months, depending on the person's body and on the HIV-test that's used. ...Read more

Dr. Shari Jackson
16 doctors shared insights

Hiv (Definition)

Hiv infection is caused by a retrovirus....This retrovirus binds to CD4 cells (for the most part). You may detect the virus by several different methods. An elisa test (enzyme linked immunosorbent assay). You may also detect it by doing a test referred to as a western blot (a gel protein electrophoresis). Thirdly by pcr (polymerase chain reaction) which ...Read more


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What is the most accurate type of HIV test?

What is the most accurate type of HIV test?

Serology: A standard serology (elisa, with western blot confirmation of positive results). ...Read more

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If we are to put a % figure, how accurate will be 4th gen ELISA test for HIV after lets say 4 weeks, then 6 weeks and then 8 weeks?

If we are to put a % figure, how accurate will be 4th gen ELISA test for HIV after lets say 4 weeks, then 6 weeks and then 8 weeks?

Why do you ask?: If you are this worried, then you should be willing to ask your physician for a pcr / nucleic acid assay for HIV instead. The modest price you'll probably end up having to pay is nothing compared with the peace of mind it'll bring you. Afterwards, get with your physician ; get some guidance about your generalized health anxiety -- you know you'll benefit from this also. ...Read more

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What is the difference between elfa and ELISA test for hiv. Are both of them accurate, lest say testing after 1 year of risky behaviour?

What is the difference between elfa and ELISA test for hiv. Are both of them accurate, lest say testing after 1 year of risky behaviour?

ELISA - initial test: Enzyme-linked immunosorbent asay (elisa) is the first one used to detect infection with hiv; if positive, it should be repeated for confirmation. Likelihood of the test being false after 1-3 months of exposure very unlikely. Enzyme linked fluouresecent assay (elfa) is a slightly newer but very similar test, but may detect HIV antibodies somewhat earlier (95% in 28 days, essentially 100% in 90 days. ...Read more

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Is ELISA test for HIV 1 & 2 antibodies conclusive after 8-9 weeks of exposure? Does NON-REACTIVE mean NEGATIVE?

ELISA for HIV: The ELISA test is not conclusive after any amount of time. The ELISA is a screening test and if it comes back positive, a Western blot will need to be done. Non-reactive is a good thing to be and it does mean that you are negative for HIV virus. Although there is a window period when you can have the HIV virus but still have a nonreactive result. Another test may be needed in that case. Suerte ...Read more

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Hi doctor. Is eli a more developed ELISA test for HIV ? And is ecl a western blot test? (if yes for ecl) i had a negative p24 at 3 month am i negative

Hi doctor. Is eli a more developed ELISA test for HIV ? And is ecl a western blot test? (if yes for ecl) i had a negative p24 at 3 month am i negative

Not Best Focus-Vague: Acronyms are too vague & not clear. However, the bigger issue is attributing too much significance to any specific test, all of which have issues of both false positives & negatives, never perfect. The larger issues are both probability, look up "bayes' theorem" & the many variables/complexity of exposure combined with individual responses over time. Before >6 months, reliability of HIV tests low. ...Read more

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My brother had a positive ELISA test for HIV followed by an indeterminate western blot. His viral load detected no virus. How confident should I be?

Pretty confident: It sounds like's he's negative and just happens to have an indeterminate wb. This happens sometimes, for reasons that are never entirely explained. However, i would recommend that he get the elisa and western blot retested one month after the first. If there has been no progression of the WB (addition of new bands), then he's definitely negative. ...Read more

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Hi doctor i just have question 14 weeks from elisa test antibody hiv-1and hiv2 was negative is that conclusive and i tested also oraquick came up negative as well. do i need further testing?

Hi doctor i just have question 14 weeks from elisa test antibody hiv-1and hiv2 was negative is that conclusive and i tested also oraquick came up negative as well. do i need further testing?

Both are conclusive: Since the ELISA included HIV2 as well as HIV1, it is a "third generation" antibody test. Those tests are virtually 100% conclusive by 6-8 weeks after exposures. (No need to wait 3 months; that's old news.) The Oraquick oral fluids test is conclusive by 3 months. Therefore, your tests are definitive and prove you don't have HIV, assuming no more recent exposures. You don't need any more tests. ...Read more

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Does ELISA HIV test detect type o or other type of hiv?

HIV screening: An ELISA is often done as an initial HIV screening test. It is designed to have high sensitivity for most types of HIV in the gene real population, but does have some false positives. That being said, there are some types of HIV, e.g. type O, that are unusual outside of Cameroon or West Africa, but are not recognized by usual antibody tests. Check with an I.D. specialist for your area and test. ...Read more

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My ELISA HIV test came up negative after 1month exposure. How accurate is this test?

90%: There's a period of time after a person is infected during which they won't test positive. This is called the “hiv window period.” The window period can be from 9 days to 3-6 months, depending on the person's body and on the HIV-test that's used. ...Read more

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What do you suggest if my ELISA HIV test came up negative after 1month exposure. is this accurate?

What do you suggest if my ELISA HIV test came up negative after 1month exposure. is this accurate?

HIV testing: No. It is not accurate in any way. It is completely invalid as a test this close to the exposure and an inappropriate test to order for a one time exposure that close to the incident. The only accurate test one month after exposure to HIV is the HIV RNA Test. ...Read more

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If an ELISA HIV test is negetive at two months.... What are the chances of the result being negetive after three months test?

If an ELISA HIV test is negetive at two months.... What are the chances of the result being negetive after three months test?

HIV Testing: There is absolutely no correct answer to your question. I know you are asking this over and over. Are you hoping for a different answer? Since you continue to ask about the HIV antibody testing there is no right answer. But, by three months the majority of those who are positive will test positive. I do not think there are verifiable statistics for sixty days. ...Read more

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Can healthy people get false positive ELISA HIV test? If so why?

Yes, it is possible: Some people may have a biologically false-positive test for hiv, when tested by elisa. Such people usually have a negative or indeterminate confirmatory test, the western blot. That usually happens because of some antibodies that were directed against other organisms recognizing part of hiv. If there is any question, a qualitative HIV dna pcr may put the question to rest. ...Read more

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Can healthy individuals get false positive ELISA HIV test? Final question. Does it always mean there's something wrong with someone if they get this.

Yes and No: Healthy people can get a positive screening test for hiv. Screening tests have high sensitivity but not very high specificity. Many issues can cause a false positive including flu vaccination. Mostly the cause of false positive is not known and it is not because there is anything wrong with the person who gets a false positive test. ...Read more

Dr. Lisa Oldson
370 doctors shared insights

Hiv Test (Definition)

There are several ways to detect the HIV virus. The first is by antibodies. This includes eia (enzyme immuno assay). The rapid HIV tests are these types. The second way detects proteins on the viral coat (western blot). This is a gel plate electrophoresis. Thirdly there is pcr (polymerase chain reaction). This detects and amplifies dna allowing a viral ...Read more


Dr. Ed Friedlander
1,461 doctors shared insights

Human Immunodeficiency Virus (Definition)

Human immunodeficiency virus (HIV) is a lentivirus (a member of the retrovirus family) that causes acquired immunodeficiency syndrome (aids), [1][2] a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections ...Read more